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目的通过观察凋亡相关基因Survivin在哈萨克族食管癌癌组织及其癌旁组织中的表达来探讨Survivin哈萨克族食管癌发生中的作用。方法采用RT-PCR方法检测Survivin基因mRNA在20例哈萨克族食管癌组织及其癌旁组织标本中的表达,选取mRNA水平差异组织标本采用Western-bolt方法检测其在蛋白水平的表达情况。结果Survivin基因mRNA在20例癌组织中有10例表达,阳性率为50%,癌旁组织中仅有4例表达,癌组织与其对应的癌旁组织相比,Survivin基因的表达量均明显高于癌旁组织。Survivin蛋白的表达在癌组织与癌旁组织中无明显差异。结论提示Sur-vivin基因的异常表达主要在转录水平,其对哈萨克族食管癌发生发展过程中的作用有待于进一步研究。
Objective To investigate the role of Survivin in the occurrence of esophageal cancer in Kazakh by observing the expression of Survivin in Kazakh esophageal cancer and its adjacent tissues. Methods RT-PCR was used to detect the expression of Survivin mRNA in 20 cases of Kazakh esophageal cancer tissues and adjacent normal tissues. Western blotting was used to detect the protein expression of Survivin mRNA. Results Survivin gene mRNA was detected in 10 of 20 cases of cancerous tissues, with a positive rate of 50% and only 4 cases of paracancerous tissues. The expression of Survivin gene was significantly higher in cancer tissues than that in paracancerous tissues In paracancerous tissues. The expression of Survivin protein in cancer tissue and paracancerous tissues showed no significant difference. The results suggest that the abnormal expression of Sur-vivin gene mainly at the transcriptional level, and its role in the occurrence and development of Kazakh esophageal cancer needs further study.