用溶壁酶制备出平菇原生质体，经过原生质体再生，获得３５６个无性系菌株。从中随机选出３１个原生质体Ｆ１代无性系，２５个原生质体Ｆ２代无性系进行一些性状研究。所测的３１个无性系Ｆ１代菌株都产生了新的遗传变异。所测的５６个无性系中有３１个茵株的生物效率显著高于亲株。所以，原生质体再生无性系的变异，可以丰富食用菌的品种资源，为高技术育种开辟了一条新途径。 Protoplast of Pleurotus ostreatus was prepared by lywallzyme, and 356 clones were obtained after protoplast regeneration. A total of 31 protoplasts from F1 clones were randomly selected and 25 from F2 clones of protoplasts. All 31 tested F1 clones had new genetic variation. The bio-efficiency of 31 of the 56 clones tested was significantly higher than that of the parent strain. Therefore, the variation of protoplast regeneration clones can enrich the variety resources of edible fungi and open up a new way for high-tech breeding.