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目的探讨自体颌下腺移植治疗角结膜干燥症后同种异体板层角膜移植术后植片存活情况和CD25+细胞在角膜排斥植片的表达情况。设计实验研究。研究对象健康成年新西兰兔20只。方法 20只实验动物用摘除泪腺和Harder腺的方法制成干眼模型,随机分两组:实验组行自体颌下腺移植;对照组不处理。移植腺体成活后两组都行同种异体板层角膜移植术。主要指标角膜植片存活时间和CD25+细胞在角膜植片表达情况。结果实验组角膜植片存活时间(37.0±5.4天)明显长于对照组(21.3±4.3天)(t=6.495,P<0.001),两组植片CD25表达情况(实验组:22.80±1.61个/5个倍视野;对照组:23.05±1.87个/5个高倍视),无显著差异(t=0.287,P>0.05)。结论颌下腺移植术后角膜植片失败主要原因是免疫排斥反应、血管化高危植床及泪液分泌不稳定。唾液泪液对角膜植片无明显不良影响。
Objective To investigate the survival of allogeneic lamellar keratoplasty after autologous submandibular gland transplantation and the expression of CD25 + cells in corneal allografts. Design experiment research. Twenty healthy adult New Zealand rabbits were studied. Methods Twenty rabbits were randomly divided into two groups: experimental submandibular gland transplantation and control group without treatment. Allograft lamellar keratoplasty was performed in both groups after transplantation of gland. The main indexes of corneal graft survival time and CD25 + cells in corneal graft expression. Results The corneal graft survival time (37.0 ± 5.4 days) in the experimental group was significantly longer than that in the control group (21.3 ± 4.3 days) (t = 6.495, P <0.001) 5 times field of view; control group: 23.05 ± 1.87 / 5 high power), no significant difference (t = 0.287, P> 0.05). Conclusion The main reasons of corneal graft failure after submandibular gland transplantation are immune rejection, vascularization high-risk graft and tear secretion instability. Saliva tears on the corneal graft no significant adverse effects.