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目的研究荷叶的乙醇提取物(LLE)、总黄酮部位(TF)和总生物碱部位(TA)对P-糖蛋白(P-gp)功能的影响。方法用Caco-2细胞模型,将实验分为阴性对照组(罗丹明-123)、阳性对照组(维拉帕米)、3个药物(LLE、TF、TA)实验组,以具有荧光特性的罗丹明-123为探针底物,以维拉帕米作为阳性抑制药,用流式细胞技术检测在荷叶提取物作用下Caco-2细胞内的荧光强度变化。结果 LLE和TA能浓度依赖性地增加Caco-2细胞内的荧光强度,100μg·mL~(- 1)LLE和TA的增加幅度分别为33.98%,114.19%,与阴性对照组比较,差异均有统计学意义(P<0.05),显示出显著的P-gp抑制作用。100μg·mL~(-1)TF在细胞内的荧光强度增加8.39%,显示出微弱的抑制作用,与阴性对照组比较差异均无统计学意义(P>0.05)。结论荷叶提取物对P-gp有一定的抑制作用,该作用与荷叶所含的生物碱成分有关。
Objective To investigate the effect of lotus leaf ethanol extract (LLE), total flavonoid fraction (TF) and total alkaloid fraction (TA) on P-glycoprotein (P-gp) function. Methods Caco-2 cell model was used to divide the experiment into negative control group (rhodamine-123), positive control group (verapamil) and three drugs (LLE, TF, TA) Rhodamine-123 as a probe substrate, verapamil as a positive inhibitor, using flow cytometry in Caco-2 cells under the influence of lotus leaf extract fluorescence intensity changes. Results LLE and TA increased the fluorescence intensity in Caco-2 cells in a concentration-dependent manner. The increase of LLE and TA of 100 μg · mL -1 was 33.98% and 114.19%, respectively. Compared with the negative control group, Statistically significant (P <0.05), showing significant inhibition of P-gp. Fluorescence intensity of 100μg · mL -1 TF increased 8.39%, showing a weak inhibitory effect, which showed no significant difference compared with the negative control group (P> 0.05). Conclusion lotus leaf extract on P-gp have a certain degree of inhibition, the role of lotus leaf alkaloids contained in the composition.