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目的分析2012-2014年黑龙江省发热伴出疹的病原谱构成。方法应用实时荧光定量PCR(real-time PCR)方法对337例临床标本进行肠道病毒、水痘-带状疱疹病毒、风疹病毒、麻疹病毒、人类小DNA病毒B19的病原学检测。结果337例标本中病毒核酸阳性结果共232份,其中EV71阳性33份,阳性率为14.22%;CVA16阳性42份,阳性率为18.10%;其他肠道病毒阳性7份,阳性率为3.0%;CVA6阳性46,阳性率为19.83%;CVA10阳性5份,阳性率为2.2%;水痘-带状疱疹阳性69份,阳性率为29.74%;风疹阳性15份,阳性率为6.5%;麻疹阳性15,阳性率为6.5%。结论肠道病毒是黑龙江省发热伴出疹性疾病的主要的病原体,其次是水痘,再次是麻疹和风疹,肠道病毒每年流行的优势基因型不同。
Objective To analyze the composition of the pathogenic spectrum of fever and rash in Heilongjiang province during 2012-2014. Methods 337 cases of clinical specimens were tested for pathogenicity of enterovirus, varicella-zoster virus, rubella virus, measles virus and human small DNA virus B19 by real-time PCR. Results A total of 232 viral nucleic acid positive results were obtained from 337 samples, of which 33 were positive for EV71, the positive rate was 14.22%; 42 were positive for CVA16; the positive rate was 18.10%; the others were positive for other enterovirus 7, the positive rate was 3.0% CVA6 positive 46, positive rate 19.83%; CVA10 positive 5, the positive rate was 2.2%; Varicella-shingles positive 69, the positive rate was 29.74%; rubella positive 15, the positive rate was 6.5%; measles positive 15 , The positive rate was 6.5%. Conclusion Enterovirus is the main pathogen of fever and rash in Heilongjiang Province, followed by chickenpox, again with measles and rubella, and the predominant prevalence of enterovirus is different.