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旨在通过对非长绒期阿尔巴斯型绒山羊进行褪黑激素埋植,研究褪黑激素对羊绒周期性生长的调控,以期借此延长绒山羊毛囊的兴盛期,提高羊绒产量。将试验羊随机分成两组:埋植褪黑激素的试验组(T)和对照组(C),每组3只(试验组(TI、T2和T3)和对照组(C1、C2和C3)),采集绒山羊的皮肤组织进行组织切片,染色观察埋植褪黑激素对毛囊生长诱导作用。分别抽提两组样本总RNA,逆转录合成相应被标记的cDNA探针,采用Agilent绵羊的8×15K规格全基因组表达谱芯片进行杂交,筛选差异表达基因。利用实时荧光定量PCR技术进行验证。结果表明,从组织学分析可观察到埋植褪黑激素对毛囊生长具有明显的促进作用;芯片数据显示,筛选出差异表达的基因95个,其中61个表达上调、34个下调。GO分析基因数量分布情况:参与分子功能的基因占47.78%,参与生物学过程的基因占33.89%,组成细胞成分的基因占18.33%。与对照组的样本相比,埋植褪黑激素后相关基因的差异性表达涉及毛囊生长及周围皮肤附属物形态发生等生物学过程。这些差异性表达的基因为绒山羊毛囊生长及周期性调控的基因功能研究提供极有价值的参考。
The aim of this study was to investigate the regulation of melatonin on the periodontal growth of cashmere by implanting melatonin on non-cashmere goat cashmere goats in order to prolong the prosperity of cashmere goat hair follicles and improve the cashmere yield. The test sheep were randomly divided into two groups: test group (T) and control group (C) with melatonin implanted, three rats in each group (TI, T2 and T3) and control group (C1, C2 and C3) ), The skin tissues of cashmere goats were collected for tissue sections, and the induction of melatonin implantation on the growth of hair follicles was observed by staining. The total RNA was extracted from two sets of samples respectively. The corresponding labeled cDNA probes were reverse transcribed. The differential expression genes were screened by 8 × 15K genome-wide cDNA microarray from Agilent sheep. Using real-time fluorescence quantitative PCR technology to verify. The results showed that, from the histological analysis, it was observed that melatonin was implanted in the hair follicles significantly; 95 of the differentially expressed genes were screened by chip data, of which 61 were up-regulated and 34 down-regulated. GO analysis of the number of gene distribution: genes involved in molecular functions accounted for 47.78%, genes involved in biological processes accounted for 33.89%, composed of cellular components accounted for 18.33% of genes. Differences in expression of related genes after implantation of melatonin involved biological processes such as hair follicle growth and the formation of peripheral skin appendages compared to the control group. These differentially expressed genes provide a valuable reference for the study of the gene function of the cashmere goat hair follicle growth and cyclical regulation.