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为了建立细叶十大功劳叶中总生物碱的提取及含量测定方法,试验以盐酸-甲醇(1∶100)为溶剂,采用回流提取法提取细叶十大功劳叶中总生物碱,并以盐酸小檗碱为对照品,紫外分光光度法测定其含量,选定波长为345 nm。结果表明:细叶十大功劳叶中总生物碱含量以盐酸小檗碱计为3.825%,线性回归方程为A=0.063 58C-0.004 48(r=1.000 0),平均回收率为100.7%,相对标准偏差(RSD)=0.45%(n=6)。说明该含量测定方法操作简便、结果准确,可用于细叶十大功劳叶中总生物碱含量的测定。
In order to establish a method for the extraction and determination of total alkaloids from the leaves of Ten Kikuviana, the total alkaloids in Ten Kikuyu leaves were extracted by reflux extraction using hydrochloric acid-methanol (1: 100) as solvent, Berberine hydrochloride as a reference substance, its content was determined by UV spectrophotometry, the selected wavelength was 345 nm. The results showed that the total alkaloid content in the leaves of the leaves was 3.825%, the linear regression equation was A = 0.063 58C-0.004 48 (r = 1.000 0) with the average recovery of 100.7% Standard deviation (RSD) = 0.45% (n = 6). This method is simple, accurate and can be used to determine the content of total alkaloids in the leaves of Ten Kobo leaves.