论文部分内容阅读
曾从人源性噬菌体抗体库中筛选出１株抗人乙肝表面抗原（ＨＢｓＡｇ）的Ｆａｂ克隆，为了筛选出新的抗ＨＢｓＡｇＦａｂ段，采用抗原屏蔽法，用已得到的Ｆａｂ段封闭相应的抗原决定基，对该抗体库进行了再次筛选，得到了１株新的人抗ＨＢｓＡｇＦａｂ段克隆，经序列分析发现其轻链可变区基因来源于Ｖκ１亚群和Ｊκ４基因，重链可变区基因来源于ＶＨ１亚群和ＪＨ４基因，但在ＶＨ第７７位和第７８位氨基酸残基之间出现了７个多余的氨基酸残基，经对基因数据库检索未能查到该序列的来源，但显然这段序列未影响抗体的抗原结合活性。
In order to screen a new anti-HBsAgFab fragment, a Fab clone of human anti-human hepatitis B surface antigen (HBsAg) was screened from the human phage antibody library. The antigenic screening method was used to screen the corresponding antigens with the obtained Fab fragment. A new strain of human anti-HBsAgFab was cloned. Sequence analysis revealed that the light chain variable region genes were derived from the Vκ1 subgroup and the Jκ4 gene, and the heavy chain variable region gene sources The VH1 subgroup and the JH4 gene, but there are 7 extra amino acid residues between the 77th and the 78th amino acid residues of VH, and the origin of this sequence can not be found by searching the gene database, but obviously Segment sequences did not affect the antigen binding activity of the antibody.