论文部分内容阅读
建立了采用高效液相色谱(HPLC)定量测定桦褐孔菌子实体和发酵菌丝体中白桦脂醇、麦角甾醇、胆甾醇、羊毛甾醇、豆甾醇和谷甾醇含量的方法。色谱条件:以C18柱进行分离,流动相为不同浓度梯度的水-甲醇(0-10min,体积比为10∶90;10-40min,体积比为3∶97),流速为1.4mL/min,检测波长为202nm,整个分析在40min内完成。结果表明所建立的方法具有很好的重复性和回收率。甾类化合物分析测定的日内相对标准偏差为2.10～2.94%(n=5),在0.4～4.8μg范围内有很好的线性关系。白桦脂醇、麦角甾醇、胆甾醇、羊毛甾醇、豆甾醇和谷甾醇的回收率分别为100.05%～100.72%,99.31%～101.04%,97.52%～101.63%,96.61%～100.08%,96.21%～100.76%和100.04%～100.51%。本方法可快速、准确地定量测定桦褐孔菌子实体和发酵菌丝体中的甾类化合物。
A method for the quantitative determination of betulin, ergosterol, cholesterol, lanosterol, stigmasterol and sitosterol in fruit bodies of inonotus obliquus and fermentation mycelium was established using high performance liquid chromatography (HPLC). Chromatographic conditions: The separation was performed on a C18 column. The mobile phase consisted of water-methanol with different concentration gradients (0-10 min, volume ratio of 10:90, 10-40 min, volume ratio of 3:97), and flow rate of 1.4 mL/min. The detection wavelength is 202 nm and the entire analysis is completed within 40 min. The results show that the established method has good repeatability and recovery. The intraday relative standard deviation of the analysis of terpenoids was 2.10 to 2.94% (n=5), and there was a good linearity in the range of 0.4 to 4.8 μg. The recoveries of betulin, ergosterol, cholesterol, lanosterol, stigmasterol, and sitosterol were 100.05% to 100.72%, 99.31% to 101.04%, 97.52% to 101.63%, 96.61% to 100.08%, and 96.21%, respectively. 100.76% and 100.04% to 100.51%. The method can rapidly and accurately quantify the terpenoids in the fruit bodies and fermentation mycelium of Inonotus obliquus.