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目的：研究胃癌中Ｐ５３、ＰＣＮＡ和ＧＳＴπ表达及意及。方法：应用免疫组织化学ＳＰ方法检测５例正常胃组织和５４例胃癌组织中Ｐ５３蛋白、增殖细胞核抗原（ＰＣＮＡ）和胎盘型谷胱甘肽Ｓ－转移酶（ＧＳＴπ）的表达水平。结果：５例正常组织中未见Ｐ５３ＰＣＮＡ和ＧＳＴπ表达。５４例胃癌组织中Ｐ５３，ＰＣＮＡ和ＧＳＴπ阳性检出率分别为６１１１％，７０３７％和６８５２％。表达阳性Ｐ５３和ＰＣＮＡ均定位于肿瘤细胞核内，ＧＳＴπ定位于胞浆。Ｐ５３、ＰＣＮＡ和ＧＳＴπ表达率与胃癌组织类型和淋巴转移关系密切。结论：检测Ｐ５３、ＰＣＮＡ和ＧＳＴπ有助于评价胃癌预后
Objective: To study the expression and significance of P53, PCNA and GSTπ in gastric cancer. Methods: The expression of P53 protein, proliferating cell nuclear antigen (PCNA) and placental glutathione S-transferase (GSTπ) were detected in 5 normal gastric tissues and 54 gastric cancer tissues by immunohistochemical SP method. Results: There was no expression of P53PCNA and GSTπ in 5 normal tissues. The positive rates of P53, PCNA and GSTπ were 61.1%, 70.7% and 68.5% respectively in 54 cases of gastric cancer. Both positive P53 and PCNA were localized in tumor nucleus, and GSTπ was located in cytoplasm. The expression rates of P53, PCNA, and GSTπ are closely related to the histological type and lymph node metastasis of gastric cancer. Conclusion: Detection of P53, PCNA, and GSTπ can help evaluate the prognosis of gastric cancer