目的:探讨罗哌卡因对肺癌A549细胞增殖、迁移和侵袭的影响及其机制。方法:2019年1月至2020年4月，将体外培养A549细胞(购自中国科学院上海细胞库)分为对照组、不同剂量[25、50、100 mg/L，罗哌卡因组(Rop组)]、乱序无意义阴性序列组(si-NC组)、si-circ_0044516组、Rop＋pcDNA组和Rop＋pcDNA-circ_0044516组，细胞计数试剂盒(CCK-8)法检测细胞增殖，Transwell检测细胞迁移和侵袭，蛋白质印迹法(Western blot)检测细胞中细胞核增殖抗原(Ki-67)、基质金属蛋白酶(MMP)-2和MMP-9蛋白表达，实时定量反转录聚合酶链反应(RT-qPCR)法检测circ_0044516和微小RNA(miRNA，miR)-198表达。双荧光素酶报告基因实验验证circ_0044516和miR-198调控关系。两组间比较行n t检验，多组间比较采用单因素方差分析，进一步两两比较采用LSD-n t检验。n 结果:不同剂量Rop组A549细胞抑制率均高于对照组[(22.54±2.11)%、(47.01±4.28)%、(65.84±4.63)%比(0.00±0.00)%，n F＝670.553，n P<0.05]，细胞迁移数[(82.08±4.60)、(63.02±4.34)、(47.94±3.85)个比(105.64±11.21)个，n F＝123.952，n P<0.05]、侵袭数[(70.94±5.08)、(53.63±4.11)、(31.01±3.34)个比(93.00±6.25)个，n F＝267.501，n P<0.05]及Ki-67(0.62±0.05、0.46±0.04、0.32±0.03比0.75±0.04，n F＝191.409，n P<0.05)、MMP-2(0.41±0.03、0.28±0.03、0.13±0.02比0.59±0.04，n F＝361.500，n P<0.05)和MMP-9(0.67±0.05、0.52±0.03、0.36±0.03比0.82±0.06，n F＝177.835，n P<0.05)的蛋白表达、circ_0044516表达均低于对照组(0.81±0.06、0.61±0.05、0.45±0.04比1.00±0.06，n F＝182.097，n P<0.05)，miR-198表达高于对照组(1.64±0.12、2.16±0.20、2.77±0.23比1.00±0.05，n F＝185.997，n P<0.05)，差异均有统计学意义。si-circ_0044516组细胞抑制率高于si-NC组[(51.27±4.11)%比(5.69±0.46)%，n t＝33.064，n P<0.05]，迁移数[(55.57±4.04)个比(107.65±10.84)个，n t＝13.506，n P<0.05]、侵袭数[(43.02±4.08)个比(92.42±7.84)个，n t＝16.768，n P<0.05)及Ki-67(0.40±0.03比0.78±0.05，n t＝19.551，n P<0.05]、MMP-2(0.21±0.03比0.58±0.04，n t＝22.200，n P<0.05)和MMP-9(0.42±0.04比0.86±0.06，n t＝18.305，n P<0.05)的蛋白表达均低于si-NC组，差异均有统计学意义。circ_0044516靶向负调控miR-198。Rop＋pcDNA-circ_0044516组细胞抑制率低于Rop＋pcDNA组[(28.57±2.49)%比(67.48±4.78)%，n t＝21.658，n P<0.05]，迁移数[(88.57±5.31)个比(46.35±4.33)个，n t＝18.486，n P<0.05]、侵袭数[(74.57±5.97)个比(28.26±2.19)个，n t＝21.848，n P<0.05]及Ki-67(0.64±0.04比0.31±0.03，n t＝19.800，n P<0.05)、MMP-2(0.46±0.04比0.12±0.02，n t＝22.808，n P<0.05)和MMP-9(0.73±0.06比0.33±0.03，n t＝17.889，n P<0.05)的蛋白表达均高于Rop＋pcDNA组，差异均有统计学意义。n 结论:Rop可能通过调控circ_0044516/miR-198轴抑制肺癌A549细胞增殖、迁移和侵袭。“,”Objective:To investigate the effect of ropivacaine (Rop) on the proliferation, migration and invasion of lung cancer A549 cells and its mechanism.Methods:The study time was from January 2019 to April 2020. A549 cells were purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences. A549 cells were cultured n in vitro and divided into control group, different doses (25, 50, 100 mg/L) Rop groups, out-of-order nonsense negative sequence(si-NC) group, si-circ_0044516 group, Rop+ pcDNA group and Rop+ pcDNA-circ_0044516 group. The cell proliferation was detected by cell counting kit-8 (CCK-8) assay, cell migration and invasion were examined by Transwell, the protein expression of proliferation cell nuclear antigen (Ki-67), matrix metalloproteinase (MMP)-2 and MMP-9 in A549 cells was detected by Western blotting, and the expression of circ_0044516 and microRNA (miRNA, miR)-198 was detected by real-time quantitative reverse transcriptase-polymerase chain reaction (RT-qPCR). The dual luciferase reporter gene experiment verified the regulatory relationship between circ_0044516 and miR-198. The two groups were compared by n t test; the comparison between multiple groups was performed by one-way analysis of variance, and the pairwise comparison between groups was performed by least significant difference n t (LSD-n t) test.n Results:Compared with the control group, the inhibition rate of A549 cells in the Rop groups with different doses increased [(22.54±2.11)%, (47.01±4.28)%, (65.84±4.63)% to (0.00±0.00)%, n F=670.553, n P<0.05], the number of migrating cells (82.08±4.60, 63.02±4.34, 47.94±3.85 vs. 105.64±11.21,n F=123.952, n P<0.05), the number of invading cells (70.94±5.08, 53.63±4.11, 31.01±3.34 vs. 93.00±6.25,n F=267.501, n P<0.05) and the protein expression of Ki-67 (0.62±0.05, 0.46±0.04, 0.32±0.03 vs. 0.75±0.04,n F=191.409, n P<0.05), MMP-2 (0.41±0.03, 0.28±0.03, 0.13±0.02 vs. 0.59±0.04,n F=361.500, n P<0.05) and MMP-9 (0.67±0.05, 0.52±0.03, 0.36±0.03 vs. 0.82±0.06,n F=177.835, n P<0.05) decreased, and the expression of circ_0044516 decreased (0.81±0.06, 0.61±0.05, 0.45±0.04 vs. 1.00±0.06,n F=182.097, n P<0.05), while the expression of miR-198 increased (1.64±0.12, 2.16±0.20, 2.77±0.23 vs. 1.00±0.05,n F=185.997, n P<0.05). Compared with the si-NC group, the inhibition rate of the si-circ_0044516 group increased [(51.27±4.11) vs. (5.69±0.46),n t=33.064, n P<0.05], the number of migrating cells (55.57±4.04 vs. 107.65±10.84,n t=13.506, n P<0.05), the number of invading cells (43.02±4.08 vs. 92.42±7.84,n t=16.768, n P<0.05) and the protein expression of Ki-67 (0.40±0.03 vs. 0.78±0.05,n t=19.551, n P<0.05), MMP-2 (0.21±0.03 vs. 0.58±0.04,n t=22.200, n P<0.05) and MMP-9 (0.42±0.04 vs. 0.86±0.06,n t=18.305, n P<0.05) decreased. The circ_0044516 negatively regulated the expression of miR-198. Compared with the Rop+ pcDNA group, the cell inhibition rate of the Rop+ pcDNA-circ_0044516 group was reduced [(28.57±2.49)% vs. (67.48±4.78)%,n t=21.658, n P<0.05], the number of migrating cells (88.57±5.31 vs. 46.35±4.33,n t=18.486, n P<0.05), the number of invading cells (74.57±5.97 vs. 28.26±2.19,n t=21.848, n P<0.05) and the protein expression of Ki-67 (0.64±0.04 vs. 0.31±0.03,n t=19.800, n P<0.05), MMP-2 (0.46±0.04 vs. 0.12±0.02,n t=22.808, n P<0.05) and MMP-9 (0.73±0.06 vs. 0.33±0.03,n t=17.889, n P<0.05) increased.n Conclusion:Rop may inhibit the proliferation, migration and invasion of lung cancer A549 cells by regulating the circ_0044516/miR-198 axis.