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1986～1987年我们在乙型脑炎(简称乙脑)流行区泉州市采集蚊虫,进行病毒分离,其中由骚扰阿蚊分离出两株病毒,经鉴定为乙脑病毒,这是我省首次从该蚊种中分离出病毒。一、材料与方法 (一)蚊标本的采集与处理:于泉州市近郊自然村的猪、牛舍内捕获吸血的蚊虫,放小蚊笼内饲养1周,待胃血消化后将其中骚扰阿蚊(简称阿蚊)集中,每30～50只为一批,按常规方法磨成悬液。 (二)蚊标本的接种:取蚊磨液分别接种C6/36细胞(伊蚊传代细胞)、BHK—21细胞(地鼠肾传代细
From 1986 to 1987, we collected mosquitoes in Quanzhou, a endemic area of Japanese encephalitis (JE), and isolated the viruses. Two viruses were isolated from the mosquitoes, and identified as JE virus. This is the first time that our province The mosquito species isolated virus. I. Materials and Methods (I) Collection and Processing of Mosquito Specimens: Blood-sucking mosquitoes were caught in pigs and cattle houses in the natural villages in the suburbs of Quanzhou. The mosquitoes were kept for one week in the mosquito cage. (Referred to as A mosquito) concentration, every 30 to 50 only for a group, according to the conventional method ground into suspension. (B) vaccination of mosquito specimens: mosquito grinding fluid were inoculated C6 / 36 cells (Aedes passage cells), BHK-21 cells (hamster kidney passage fine