目的:建立氧氟沙星氢化可的松滴耳液含量测定的方法。方法:采用RP-HPLC法测定滴耳液中氧氟沙星与氢化可的松的含量。色谱柱为Poresphere C_(18)(150 mm×4.6 mm,5μm);流动相为甲醇-0.025 mol·L~(-1)磷酸溶液(用三乙胺调pH至3.0)(55:45);流速0.8 ml·min~(-1);检测波长为240 nm;检测灵敏度为0.16AUFS,进样量20μl。结果:氧氟沙星在30～480μg·ml~(-1)浓度范围内,峰面积与浓度的线性关系良好(r=0.999 9),平均回收率为99.8%;氢化可的松在5～80μg·ml~(-1)浓度范围内,峰面积与浓度的线性关系良好(r=0.999 9),平均回收率为99.9%。结论:本方法快速、简便,精密度好,重复性好,可作为氧氟沙星氢化可的松滴耳液的含量测定方法。 Objective: To establish a method for determination of ofloxacin hydrocortisone ear drops. Methods: Determination of ofloxacin and hydrocortisone in ear drops by RP-HPLC. The chromatographic column was Poresphere C 18 (150 mm × 4.6 mm, 5 μm). The mobile phase was methanol-0.025 mol·L -1 phosphoric acid solution (adjusted to pH 3.0 with triethylamine) (55:45) The flow rate was 0.8 ml · min ~ (-1), the detection wavelength was 240 nm, the detection sensitivity was 0.16 AUFS and the injection volume was 20 μl. RESULTS: ofloxacin showed a good linearity (r = 0.999 9) with an average recovery of 99.8% at a concentration range of 30 ~ 480μg · ml ~ (-1) The linear relationship between peak area and concentration was good (r = 0.999 9) in the concentration range of 80μg · ml ~ (-1) with an average recovery of 99.9%. Conclusion: The method is rapid, simple, accurate, reproducible and can be used as a method for the determination of ofloxacin hydrocortisone ear drops.