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目的观察血管内皮细胞生长因子(vascular endothelial growthfactor,VEGF)诱导大肠癌 HT-29细胞转移及其对粘附作用的影响.方法采用~3H-TdR 掺入及鼠尾胶粘附实验测定 VEGF 对大肠癌 HT-29细胞同质性和异质性粘附作用以及 Bodyen-Chamber观察 VEGF 诱导大肠癌细胞转移.结果 1,5mg/L VEGF 诱导 HT-29细胞60,90min ~3H-TdR 掺入实验(cpm)分别为1759±289,1380±329和3124±226,2246±273,10 mg/L VEGF 诱导 HT-29细胞60,90,120min ~3H-TdR 掺入实验分别为1232±201,2338±333,2237±237,显著低于对照组60,90,120min 的2184±336,3560±255,4337±377,(P<0.05或0.01).5,10 mg/L VEGF 诱导 HT-29细胞60min 鼠尾胶粘附实验 A 值为0.263±0.021,0.238±0.034;1,5,10mg/L VEGF 诱导 HT-29细胞90,120min 鼠尾胶粘附实验 A 值分别为0.269±0.023,0.373±0.083.0.393±0.081和0.371±0.061,0.390±0.074,0.433±0.122,分别高于对照组的0.130±0.025,0.143±0.036,0.210±0.028,(P<0.05或0.01).用 VEGF 1,5,10mg/L培养5 h,下室浸润的肝癌细胞数分别为(5.75±1.00,17.17±2.38,10.33±0.88)×10~7/L,分别高于对照组(1.58±0.38)×10~7/L(P<0.05或0.01).结论 VEGF 可以促进大肠癌 HT-29细胞转移,与 VEGF 增加细胞异质性粘附作用以及降低大肠癌细胞的同质性粘附作用有关细胞.
Objective To observe the effect of vascular endothelial growth factor (VEGF) on the metastasis of colorectal cancer HT-29 cells and its effect on the adhesion.Methods The 3H-TdR incorporation and adhesion of rat tail colloids HT-29 cell homogeneity and heterogeneous adhesion of HT-29 cells and Bodyen-Chamber observation of VEGF-induced colorectal cancer cell metastasis.Results 1, 5mg / L VEGF induced 60,90min ~ 3H-TdR incorporation in HT-29 cells cpm) were 1759 ± 289, 1380 ± 329 and 3124 ± 226, 2246 ± 273 and 10 mg / L, respectively. The incorporation of 60,90,120min ~ 3H-TdR into HT-29 cells induced by VEGF was 1232 ± 201,2338 ± 333 , 2237 ± 237, significantly lower than 2184 ± 336, 3560 ± 255, 4337 ± 377 (P <0.05 or 0.01) at 60, 90, The A value of adhesive adhesion test was 0.263 ± 0.021,0.238 ± 0.034. The A values of tail adhesive adhesion of HT-29 cells induced by VEGF at doses of 1, 5 and 10 mg / L for 90 and 120min were 0.269 ± 0.023,0.373 ± 0.083.0.393 ± 0.081 and 0.371 ± 0.061,0.390 ± 0.074,0.433 ± 0.122, respectively, higher than the control group 0.130 ± 0.025,0.143 ± 0.036,0.210 ± 0.028 (P <0.05 or 0.01) with VEGF 1,5,10mg / L culture 5 h, The numbers of infiltrating hepatocellular carcinoma cells were (5.75 ± 1.00,17.17 ± 2.38,10.33 ± 0.88) × 10 ~ (7) / L, respectively, which were significantly higher than those in control group (1.58 ± 0.38 × 10-7 / L, P <0.05 or 0.01 ) Conclusion VEGF can promote the metastasis of colorectal cancer HT-29 cells, and increase the cell heterogeneous adhesion with VEGF and reduce the homogenate adhesion of colorectal cancer cells.