目的 :探讨有丝分裂原活化的蛋白激酶级联信号通路的关键效应分子——核糖体 S6蛋白激酶 (ribosom al S6 ki-nase,RSK)与肝纤维化发生的关系。 方法 :采用皮下注射二甲基亚硝胺 (dimethylnitrosamine,DMN)的方法制备大鼠肝纤维化模型 ,根据注射时间的不同 ,获取组织标本 ,行 H- E和 VG染色。明确纤维化分期后 ,进一步利用 Northern印迹及免疫组化方法 ,观察 RSK在肝纤维化进程中的表达变化情况。 结果 :RSK在正常大鼠肝脏中 ,仅在中央静脉周围的肝血窦有少量表达 ,而随着纤维化的进展 ,其表达量进行性增加 ;在纤维化肝脏 ,RSK主要分布于汇管区间质中 ,肝细胞未见染色。结论 :RSK在肝纤维化进程中持续上调 ,可能在肝纤维化的发生中起重要作用 Objective: To investigate the relationship between ribosomal-S6 kinase (RSK), a key effector of mitogen-activated protein kinase cascade, and hepatic fibrosis. Methods: Rat model of hepatic fibrosis was induced by injecting dimethylnitrosamine (DMN) subcutaneously. Tissue samples were taken from the rats according to the time of injection. H-E and VG staining were performed. After definite fibrosis staging, further use of Northern blot and immunohistochemical methods to observe the change of RSK expression in the process of liver fibrosis. Results: In normal rat liver, RSK only expressed a small amount of hepatic sinusoid around the central vein, while the expression of RSK increased progressively with the progress of fibrosis. In fibrosis liver, RSK mainly distributed in the portal area Quality, no staining of liver cells. Conclusion: RSK is continuously up-regulated in the process of hepatic fibrosis and may play an important role in the development of hepatic fibrosis