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本研究用N-甲基-N-亚硝基脲处理“Kinmaze”水稻品种可育卵细胞,从中得到11个联会消失突变体。分别命名为MM-1,MM-3,MM-5,MM-8,MM-9,MM-10,MM-11,MM-13,MM-14,MM-15和MM-18。这些种子育性分别是44.7±8.6,76.4±5.2,82.5±4.6,53.4±6.8,55.O±6.2,60.9±9.6,40.9±4.4,34.2士8.9,75.2士5.8,1.7±0.6和68.9±4.4,正常植株种子育性是94.9±3.8。所有突变体植株育性均比正常植株偏低,只有MM-5和MM-9植株高度比正常植株矮,其余都和正常植株相近。这些突变体花粉母细胞用1:3醋酸酒精溶液固定24小时后貯存在70%酒精中置冰箱内,染色体
In this study, fertile egg cells of “Kinmaze” rice varieties were treated with N-methyl-N-nitrosourea, and 11 association disappearing mutants were obtained. They are named MM-1, MM-3, MM-5, MM-8, MM-9, MM-10, MM-11, MM-13, MM-14, MM-15 and MM- 18, respectively. The seed fertility was 44.7 ± 8.6, 76.4 ± 5.2, 82.5 ± 4.6, 53.4 ± 6.8, 55 ± 6.2, 60.9 ± 9.6, 40.9 ± 4.4, 34.2 ± 8.9, 75.2 ± 5.8, 1.7 ± 0.6 and 68.9 ± 4.4, normal plant seed fertility is 94.9 ± 3.8. The fertility of all the mutant plants was lower than that of the normal plants, only the height of MM-5 and MM-9 plants was shorter than the normal plants, and the rest were similar to the normal plants. These mutant pollen mother cells were fixed in 1: 3 acetic alcohol solution for 24 hours and stored in a 70% alcohol-freezer. Chromosome