论文部分内容阅读
目的 研究复方党参对模拟高原低氧条件下大鼠脑神经细胞凋亡基因表达谱的作用 ,从中药与生物体基因网络相互作用的角度 ,探讨复方党参的作用机制。方法 将Wistar大鼠置于模拟海拔 70 0 0m的低压氧舱建立高原低氧大鼠脑细胞凋亡模型 ,于上舱前 7d开始以复方党参液灌胃给药。提取空白对照组、低氧 8d对照组、常氧复方党参组及低氧 8d复方党参组大鼠脑组织总RNA ,经逆转录合成cDNA探针 ,用 [α 3 2 P]标记探针 ,各自与AtlascDNAArray膜杂交 ,经严格的冲洗后用磷屏检cDNA表达水平。获得的基因表达谱数据集采用自组图 (SOMs)法分析。结果 结果表明在模拟高原低氧条件下 ,导致神经细胞凋亡的基因 (p5 3、bax、bad、bok、Hsp6 0 )聚为一类 (即表达行为相似 )。而具有抗凋亡功能的基因 (bcl 2、bcl xl、Hsp70、Hsp2 7、Hsp90、Hsc70、Rad)聚为一类。 结论 低氧可以启动细胞凋亡的线粒体途径 ,导致细胞死亡 ;而复方党参以凋亡相关基因网络中的多种成分为靶点 ,从不同层次上发挥抑制细胞凋亡的作用
Objective To study the effect of Codonopsis Pilosula on the expression of apoptotic genes in rat brain neurons under hypobaric conditions, and to explore the mechanism of action of Codonopsis pilosula from the perspective of the interaction between Chinese medicine and organisms. METHODS Wistar rats were placed in a hypobaric chamber at a simulated altitude of 700Om to establish a rat model of cerebral hypoxia of high altitude hypoxia. The compound dangshen solution was administered intragastrically 7 days before the upper chamber. Total RNA was extracted from the brain tissue of rats in the blank control group, hypoxia 8d control group, normoxic compound dangshen group and hypoxic 8d compound dangshen group. cDNA probes were synthesized by reverse transcription and labeled with [α 3 2 P] probes. After hybridization with the Atlas cDNA array membrane, the expression level of the cDNA was checked using a phosphor screen. The acquired gene expression profile data sets were analyzed using the self-organized map (SOMs) method. Results The results showed that genes that cause neuronal apoptosis (p53, bax, bad, bok, Hsp60) clustered into a single group (i.e., expression behavior was similar) under hypobaric hypoxic conditions. The genes with anti-apoptotic function (bcl 2, bcl xl, Hsp70, Hsp2 7, Hsp90, Hsc70, Rad) are clustered together. Conclusion Hypoxia can initiate the mitochondrial pathway of apoptosis and lead to cell death. Compound Dangshen uses multiple components of apoptosis-related gene networks as targets to inhibit apoptosis from different levels.