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目的优化蒙药赤芍防治心血管系统疾病的有效物质群的提取纯化工艺。方法采用香草醛-浓硫酸比色法,以赤芍总苷为指标,考察提取工艺和大孔树脂纯化工艺。结果最佳提取工艺为溶剂70%乙醇、提取时间4 h,溶剂量200 ml,提取次数2次;纯化工艺为3BV/h吸附速率吸附,再用30%乙醇洗脱,流速为3BV/h。结论该方法简单可行,能较好的提取纯化赤芍防治心血管系统疾病的有效物质群。
OBJECTIVE: To optimize the extraction and purification of effective substances of Mongolian medicine, Radix Paeoniae Rubra in the prevention and treatment of cardiovascular diseases. Methods Vanillin - concentrated sulfuric acid colorimetric method, the total glycosides of paeony root as an index to examine the extraction process and macroporous resin purification process. Results The optimal extraction conditions were as follows: 70% ethanol, extraction time 4 h, solvent amount 200 ml and extraction times 2 times; the purification process was adsorption at 3BV / h and then 30% ethanol, the flow rate was 3BV / h. Conclusion The method is simple and feasible, which can extract and purify the effective substance group of purifying peonye against cardiovascular diseases.