论文部分内容阅读
目的:选用骨保护素(osteoprotegerin,OPG)基因敲除的骨质疏松小鼠模型,观察阿仑膦酸钠及仙灵骨葆对其骨骼肌的作用。方法:将2个月龄的OPG基因敲除小鼠及正常小鼠随机分为安慰剂(正常组)、阿仑膦酸钠及仙灵骨葆组,予安慰剂或药物处理12周。股直肌行HE染色后观察其形态,并采用实时PCR检测股直肌重链肌球蛋白mRNA的表达情况。结果:OPG基因敲除小鼠的股直肌单位面积骨骼肌纤维数目明显增加(+12.94%,P=0.028),肌纤维横截面积明显减小(-23.0%,P=0.004),股直肌中肌球蛋白重链mRNA的表达水平显著降低(P<0.01);而阿仑膦酸钠及仙灵骨葆处理后能明显减少单位面积骨骼肌纤维数目(-14.64%,P=0.022;-9.96%,P=0.014),增加骨骼肌纤维横截面积(+24.45%,P=0.011;+23.3%,P=0.002),同时能够增加小鼠股直肌肌球蛋白重链mRNA的表达水平(P<0.01)。结论:阿仑膦酸钠及仙灵骨葆能明显改善OPG基因敲除所致骨质疏松小鼠骨骼肌的退化,其作用机制可能与改善骨密度或中药的植物雌激素作用有关。
Objective: To investigate the effects of alendronate and xianlinggubao on skeletal muscle in a mouse model of osteoporosis induced by osteoprotegerin (OPG) gene knockout. Methods: 2-month-old OPG knockout mice and normal mice were randomly divided into placebo group (normal group), alendronate and Xianlinggubao group, placebo or drug treatment for 12 weeks. The rectus femoris was observed by HE staining and the expression of myosin mRNA in the rectus femoris was detected by real-time PCR. RESULTS: The number of skeletal muscle fibers per unit area of rectus muscle was significantly increased in OPG knockout mice (+ 12.94%, P = 0.028), and the cross-sectional area of muscle fiber was significantly decreased (-23.0%, P = 0.004) Myosin heavy chain mRNA expression was significantly decreased (P <0.01); while alendronate and Xianling Gu Bao treatment significantly reduced the number of skeletal muscle fibers per unit area (-14.64%, P = 0.022; -9.96% , P = 0.014), and increased skeletal muscle fiber cross-sectional area (+ 24.45%, P = 0.011; + 23.3%, P = 0.002) 0.01). CONCLUSION: Alendronate and Xianling Gubao can significantly improve the skeletal muscle degeneration of osteoporotic mice induced by OPG knockout, and its mechanism may be related to the improvement of bone mineral density or the phytoestrogen effect of traditional Chinese medicine.