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[目的]研究并比较短期(14 d)经口摄入纳米二氧化钛(titanium dioxide,TiO_2)、微米TiO_2对幼年大鼠小肠铁和葡萄糖吸收的影响。[方法]将42只幼年(4周龄)雄性Sprague-Dawley(SD)大鼠按体重用随机数字表法分为7组,染毒组每天分别灌胃给予2、10、50 mg/kg(以体重计)的纳米TiO(2平均原始粒径24 nm)或微米TiO(2平均原始粒径120 nm),对照组灌以同体积蒸馏水。连续染毒14 d后,取小肠制作翻转肠囊模型,给予20 mmol/L葡萄糖溶液和20 mmol/L硫酸铁(FeSO_4)溶液,观察小肠对铁和葡萄糖的吸收情况。[结果]与对照组相比,经口染毒纳米、微米TiO_2 14 d后,大鼠的小肠组织无明显病理改变。各组幼年大鼠小肠对铁及葡萄糖的吸收量均有随时间增加而增加的趋势。纳米TiO_2、微米TiO_2各剂量组间大鼠小肠对铁及葡萄糖的累积吸收量与吸收速率均呈略高于对照组的趋势,但差异无统计学意义(P>0.05)。同一时间点,纳米、微米TiO_2染毒组的铁与葡萄糖累积吸收量和吸收速率均未表现出剂量-反应关系,各染毒组差异无统计学意义(F铁累积吸收量=0.674,F铁吸收速率=0.674;F葡萄糖累积吸收量=1.332;F葡萄糖吸收速率=1.545;均P>0.05)。在相同染毒剂量下,纳米TiO_2染毒组的大鼠小肠对铁及葡萄糖的吸收量、吸收速率与微米TiO_2染毒组相比,差异亦无统计学意义(均P>0.05)。[结论]短期经口摄入纳米、微米TiO_2对幼年大鼠小肠铁及葡萄糖的吸收无明显影响,且纳米、微米TiO_2的作用无明显差异。
[Objective] To study and compare the effects of short-term (14 d) oral intake of titanium dioxide (TiO_2) and micro-TiO_2 on iron and glucose uptake in small intestine of young rats. [Methods] 42 young (4 weeks old) male Sprague-Dawley (SD) rats were divided into 7 groups according to body weight by random number table. The rats in the exposure group were orally administered with 2, 10 and 50 mg / kg (2 mean primary particle size 24 nm) or TiO 2 (average primary particle size 120 nm) by weight scale. The control group was filled with the same volume of distilled water. After continuous exposure for 14 days, the small intestine was taken to make a model of overturning enteric sac, and the solution of 20 mmol / L glucose and 20 mmol / L ferric sulfate (FeSO 4) was given to observe the intestinal absorption of iron and glucose. [Result] Compared with the control group, there was no significant pathological change in the small intestine of rats after oral administration of nano-TiO 2 for 14 days. The absorption of iron and glucose in the small intestine of young rats in each group increased with time. The cumulative uptake and absorption rate of iron and glucose in the small intestine of the nano-sized TiO 2 and the micro-TiO 2 groups were slightly higher than that of the control group, but the difference was not statistically significant (P> 0.05). At the same time point, there was no dose-response relationship between iron and glucose cumulative absorption and absorption rate in the groups of nano-TiO 2 and micronized TiO 2, and there was no significant difference among the three groups (cumulative iron absorption F = 0.674, F Absorption rate = 0.674; F glucose cumulative absorption = 1.332; F glucose absorption rate = 1.545; all P> 0.05). Under the same exposure dose, the absorption and absorption rate of iron and glucose in the small intestine of the nano-TiO 2 group were not significantly different from those of the TiO 2 -treated group (all P> 0.05). [Conclusion] The short-term oral intake of nano-TiO 2 had no significant effect on the absorption of iron and glucose in the small intestine of young rats, and there was no significant difference between nano and TiO 2.