莴苣上发现一种新的核盘菌菌核病

来源 :植物病理学报 | 被引量 : 0次 | 上传用户:chinesechinese123456
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从湖北省神农架莴苣上分离到一种产菌核病原真菌。这种真菌在培养特性上同核盘菌、三叶草核盘菌和小核盘菌既存在着明显差异,又存在着相似之处。其菌核易萌发成子囊柄,但在一般散射光下柄顶端难以发育成子囊盘。对偶得的1枚子囊盘观察表明这种真菌符合核盘菌属真菌的特征,并且同核盘菌属3个近缘种的菌株不同。可溶性蛋白质和多种酶同功酶电泳分析结果表明这种真菌不同于核盘菌属的3个常见种,但同它们亲缘关系较近。这种新菌核病的初侵染来源是菌核萌发产生的菌丝,通过病健接触构成再侵染,在病组织上形成菌核越冬越夏。离体和活体致病性测定结果表明这种真菌只能侵染莴苣,不能侵染油菜、小白菜、萝卜和胡萝卜。 From the Shennongjia lettuce in Hubei Province isolated from a production of nuclear pathogenic fungi. This fungus in culture with the same characteristics of Sclerotinia, Crab Flavia and Sclerotinia there are significant differences, there are similarities. The sclerotia is easy to germinate into a capsulotomy, but the top of the stalk is hard to develop into an ascosphere in the general scattering light. An even observation of 1 ascus showed that this fungus was consistent with the fungus of the genus Sclerotinia and differed from the strains of 3 closely related species of the genus Scirporum. Soluble protein and enzyme isozyme electrophoresis analysis showed that this fungus is different from the three common species of Sclerotinia, but with their close genetic relationship. The primary source of this new sclerotinosis is the mycelium germinated by the sclerotia, which forms a re-infection through disease-health contacts, forming sclerotia on sick tissues over winter. In vitro and in vivo pathogenicity assays indicated that this fungus infested lettuce only and did not infect rape, cabbage, radish and carrot.
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