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目的探讨3种肺炎支原体(mycoplasma pneumoniae,MP)快速检测方法即明胶颗粒凝集法(PAT)、胶体金免疫层析法(GICA)和酶联免疫吸附法(ELISA)法的各自临床诊断价值和优势,为临床合理选择使用这几种方法提供实验依据。方法同时用3种方法对临床疑似病人血清进行同步检测,采用四格表法对检测结果进行统计分析。结果 GICA与ELISA对MP诊断的敏感性和特异性高度一致;但这2种方法与PAT诊断结果的一致性较差。结论相对于ELISA方法,GICA法由于其经济、有效,更适合MP的早期快速筛查;虽然GICA/ELISA法与PAT法诊断结果的一致性较差,但它们的联合使用有助于快速判断MP感染时期,可为该病的治疗方案提供实验依据。
Objective To investigate the clinical diagnostic value and advantages of three rapid detection methods of Mycoplasma pneumoniae (MP), namely gelatin particle agglutination (PAT), colloidal gold immunochromatography (GICA) and enzyme-linked immunosorbent assay (ELISA) , To provide experimental basis for the clinical rational use of these methods. Methods Three kinds of methods were used to detect the serum of clinical suspect patients at the same time. Statistical analysis was made on the test results by using the four-grid table method. Results The sensitivity and specificity of GICA and ELISA for the diagnosis of MP were highly consistent. However, the consistency between the two methods and the diagnosis of PAT was poor. Conclusion Compared with the ELISA method, GICA method is more suitable for early rapid screening of MP due to its economy and effectiveness. Although the consistency between the GICA / ELISA method and the PAT method is poor, the combined use of GICA / Infected period, can provide experimental evidence for the treatment of the disease.