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目的观察宫颈病变妇女人乳头状瘤病毒16/18(HPV16/18)、单纯疱疹病毒2型(HSVⅡ)的感染情况,分析和探讨HPV 16/18及HSVⅡ协同感染与宫颈病变的相关性。方法选择2013年1~12月于海西州人民医院妇科就诊的217例患者,其中正常宫颈20例、宫颈炎55例、宫颈上皮内瘤变(CIN)117例、宫颈癌25例,采用实时荧光定量PCR法分别检测HPV16/18和HSVⅡ,比较HPV16/18和HSVⅡ的感染情况,并分析HPV16/18及HSVⅡ协同感染与宫颈病变的相关性。结果宫颈癌组HPV16/18和HSVⅡ感染阳性检出率显著高于正常宫颈组、宫颈炎组及CIN组,而CIN组HPV16/18和HSVⅡ感染阳性检出率显著高于正常宫颈组及宫颈炎组,差异均有统计学意义(P<0.05);各宫颈病变患者主要为HPV16/18感染,且病情越严重,HPV16/18及HSVⅡ感染阳性检出率越高。与单一的HPV16/18、HSVⅡ感染相比,除宫颈炎患者外,CIN和宫颈癌患者均出现HPV16/18和HSVⅡ混合感染,但混合感染的发生率显著低于单一的HPV16/18、HSVⅡ感染,差异有统计学意义(P<0.05)。结论 HPV16/18、HSVⅡ协同感染可增加宫颈癌的患病率,与宫颈癌的发生及发展有密切的关系,对高风险人群进行筛查可提高宫颈癌的早期诊断率及治疗率。
Objective To observe the infection of human papillomavirus 16/18 (HPV16 / 18) and herpes simplex virus 2 (HSV Ⅱ) in women with cervical lesions and to analyze the correlation between HPV 16/18 and HSV Ⅱ co-infections and cervical lesions. Methods A total of 217 patients with gynecological examination in Haixi People’s Hospital from January 2013 to December 2013 were selected. Among them, 20 cases were normal cervix, 55 cases were cervicitis, 117 cases were cervical intraepithelial neoplasia (CIN) and 25 cases were cervical cancer. Real-time fluorescence HPV16 / 18 and HSVⅡwere detected by quantitative polymerase chain reaction (PCR), and the infection status of HPV16 / 18 and HSVⅡwas compared. The correlation between HPV16 / 18 and HSVⅡco-infection and cervical lesions was analyzed. Results The positive rates of HPV16 / 18 and HSV Ⅱ infection in cervical cancer were significantly higher than those in normal cervix, cervicitis and CIN, but the positive rates of HPV16 / 18 and HSV Ⅱ infection in CIN were significantly higher than those in normal cervix and cervix (P <0.05). The majority of cervical lesions were HPV16 / 18 infection, and the more severe the disease was, the higher the positive rate of HPV16 / 18 and HSV Ⅱ infection was. Compared with single HPV16 / 18 and HSVⅡinfection, HPV16 / 18 and HSVⅡinfection occurred in both CIN and cervical cancer patients except for cervicitis, but the incidence of mixed infection was significantly lower than that of single HPV16 / 18 and HSVⅡ , The difference was statistically significant (P <0.05). Conclusion The synergistic infection with HPV16 / 18 and HSV Ⅱ can increase the prevalence of cervical cancer and have a close relationship with the occurrence and development of cervical cancer. Screening of high-risk groups can improve the early diagnosis and treatment rate of cervical cancer.