论文部分内容阅读
目的检测根管再治疗患者根管内白色假丝酵母菌、变形链球菌、古细菌三种细菌的检出率情况,并分析再治疗患者根管内白色假丝酵母菌、变形链球菌、古细菌三种细菌与临床症状之间的关系。方法临床选取89名根管再治疗患者,通过记录患牙的临床相关体征、症状等信息,去除根管内原有充填物,插入无菌纸尖60 s,与H锉刮取物一同放入EP管中,提取基因组DNA,通过PCR反应检测白色假丝酵母菌、变形链球菌、古细菌三种细菌的16S RNA,检测根管再治疗患者根管内白色假丝酵母菌、变形链球菌、古细菌三种细菌的检出率情况,并分析再治疗患者根管内白色假丝酵母菌、变形链球菌、古细菌三种细菌与临床症状之间的关系。结果根管再治疗患者根管内白色假丝酵母菌、变形链球菌、古细菌三种细菌的检出率分别为31.46%、29.21%、19.10%。在临床症状中叩痛病例组中的变形链球菌检出率高于无叩痛病例组(P<0.05)。结论根管再治疗患者根管内变形链球菌的检出与临床症状中的叩痛密切相关,与根尖周破坏呈正相关。根管再治疗患者根管可以检测出白色假丝酵母菌、变形链球菌、古细菌三种细菌。
Objective To detect the detection rate of Candida albicans, Streptococcus mutans and Archaebacteria in the root canal of patients undergoing root canal re-treatment, and to analyze the detection of Candida albicans, Streptococcus mutans, Relationship between bacteria and clinical symptoms. Methods Eighty-nine root canal re-treated patients were enrolled in this study. The original filling in the root canal was removed by recording the clinically relevant signs and symptoms of the affected tooth. The tip of the sterile paper was inserted for 60 seconds and placed in the EP In the tube, genomic DNA was extracted and the 16S RNAs of Candida albicans, Streptococcus mutans, and Archaebacteria were detected by PCR, and Candida albicans, Streptococcus mutans, The detection rate of bacteria in three kinds of bacteria, and analysis of re-treatment of patients with root canal Candida albicans, Streptococcus mutans, Archaebacteria three kinds of bacteria and the relationship between clinical symptoms. Results The detection rates of Candida albicans, Streptococcus mutans and Archaebacteria in the root canal re-treatment were 31.46%, 29.21% and 19.10% respectively. The detection rate of Streptococcus mutans was higher in patients with percussion pain than in those without percussion (P <0.05). Conclusion The detection of Streptococcus mutans in the root canal after root canal re-treatment is closely related to the percussion pain in clinical symptoms, and positively correlates with periapical destruction. Root canal re-treatment patients can detect Candida albicans, Streptococcus mutans, archaebacteria three kinds of bacteria.