论文部分内容阅读
目的参照国外采用吸烟导致小鼠肺肿瘤的实验模型程序和方法,应用昆明系小鼠,探索建立吸烟导致肺肿瘤模型的可行性以及应用此模型评价抗氧化剂对其干预效果。方法将200只小鼠随机分为正常对照组、烟草暴露组、槲皮素干预组和维生素E干预组,除正常对照组外,余3组均在染毒柜中被动吸烟染毒5个月,恢复4个月后处死,进行病理学和抗氧化指标的检测。槲皮素(80 mg.kg-1.d-1)和维生素E(1 00 mg.kg-1.d-1)分别加入饲料中摄入体内,连续喂养9个月。结果烟草暴露组小鼠肺肿瘤发病率为43.5%,每只肺表面的平均肿瘤结节数为1.00±0.29,明显高于对照组(11.8%,0.18±0.09)和维生素E干预组(17.9%,0.32±0.16),差异有显著性(P<0.05);维生素E干预效果最好。维生素E干预组血清中维生素E浓度明显高于其它各组,维生素E干预组活性氧(ROS)活性明显低于其它各组,差异均有显著性(P<0.05)。结论此次应用国产昆明系小鼠成功地建立了烟草暴露导致肺肿瘤模型,但其稳定性需进一步实验验证。应用昆明系小鼠所建立的肺肿瘤模型将为肺肿瘤发生过程及防治措施的研究提供了基础。
OBJECTIVE: To explore the feasibility and feasibility of tobacco smoking-induced lung tumor model in Kunming mice by referring to the experimental procedures and methods of using smoking to induce lung tumor in mice abroad. Methods 200 mice were randomly divided into normal control group, tobacco exposure group, quercetin intervention group and vitamin E intervention group. Except normal control group, all the other three groups were exposed to passive smoking for 5 months , After 4 months of recovery, sacrificed, pathological and anti-oxidative indicators were detected. Quercetin (80 mg.kg-1.d-1) and vitamin E (100 mg.kg-1.d-1) were added into the diet and fed for 9 months. Results The incidence of lung cancer was 43.5% in tobacco exposed mice and 1.00 ± 0.29 in each lung surface, which was significantly higher than that in control group (11.8%, 0.18 ± 0.09) and vitamin E intervention group (17.9% , 0.32 ± 0.16), the difference was significant (P <0.05); vitamin E intervention best. Vitamin E intervention group serum vitamin E concentration was significantly higher than the other groups, vitamin E intervention group reactive oxygen species (ROS) activity was significantly lower than the other groups, the difference was significant (P <0.05). Conclusion The Kunming mice domesticated successfully established a model of lung tumor caused by tobacco exposure, but its stability needs further experimental verification. The application of Kunming mouse model of lung cancer will provide a basis for the study of lung tumor occurrence and prevention and treatment.