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水稻重要农艺性状的基因定位研究在育种上具有重要意义。2004年在海南陵水县种植两个完全测序水稻品种日本晴与9311的F2群体及双亲,分别考察了其单株分蘖数、穗数、有效分蘖数、穗长、主穗长、抽穗期、株高和剑叶8个农艺性状3次重复的平均值。用已构建的连锁遗传图谱(Nipponbare/9311-F2遗传图谱)及Excel 2000和Mapmaker/QTL 1.1b软件对这8个性状间的相互关系和基因位点进行了分析。结果在LOD>2.0和P<0.005的条件下共检测到41个QTLs,它们分布在水稻所有染色体上,单个QTL对性状表型贡献率11.0%~46.4%,其中大于20%的有22个。对选用已测序材料为亲本构建图谱来探讨水稻农艺性状的分子基础及其育种意义进行了讨论。
Genetic mapping of important agronomic traits in rice is of great importance in breeding. In 2004, two fully sequenced F2 populations and their parents of Nipponbare and 9311 were planted in Lingshui County, Hainan Province. Their tiller number per plant, spike number, effective tiller number, ear length, main ear length, heading date, Height and the flag leaf eight agronomic traits 3 times the average of the repeats. The relationships and gene loci of the eight traits were analyzed using the constructed linkage map (Nipponbare / 9311-F2 genetic map) and Excel 2000 and Mapmaker / QTL 1.1b software. Results A total of 41 QTLs were detected under the conditions of LOD> 2.0 and P <0.005, which were distributed on all chromosomes in rice. The single QTL contributed 11.0% -46.4% of phenotypic traits, of which 22 were more than 20%. The molecular basis of selecting agronomic traits and the breeding significance of the selected materials for their parents were discussed.