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用~1H NMR研究了固氮酶在重水中催化还原乙炔的反应产物氘代乙烯.利用群对称性对~1HNMR谱图进行了归属,计算了几种可能的C_2H_2D_2结构以及C_2H_3D结构的NMH谱线频率和强度,得出了理论谱.通过理论谱与实验谱的比较,表明固氮酶在重水中催化还原乙炔的产物主要以顺式结构C_2H_2D_2为主,并含有较多的单氘代乙烯.单氕代乙烯相对乙烯的化学位移往高场移动约4.0Hz,而双氘代乙烯向高场的位移大约是单氘代乙烯的2倍左右.
1H NMR was used to study the reaction product of vinylidene catalyzed by nitrogenase in heavy water. The spectrum of ~ 1HNMR spectra was assigned by cluster symmetry. Several possible C_2H_2D_2 structures as well as the NMH spectrum of C_2H_3D were calculated And intensity, the theoretical spectrum was obtained.Comparing the theoretical spectrum with the experimental spectrum, it was shown that the products catalyzed by nitrogenase in heavy water mainly consisted of C_2H_2D_2 cis form and contained more mono-deuterated ethylene The chemical shifts of the ethylene to ethylene are shifted to about 4.0 Hz in the high field, whereas the displacement of the bidentate vinyl to the high field is about 2 times that of the mono-deuterated ethylene.