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目的:探讨蛇黄乳膏防治湿疹止痒作用的相关机制。方法:取小鼠随机分组,即对照组、模型组、艾洛松组、蛇黄乳膏各浓度组,采用2,4-二硝基氟苯(DNFB)致小鼠迟发性变态反应;用二甲苯致小鼠耳廓肿胀计算出肿胀抑制率;用角叉菜胶致小鼠足肿胀观察足肿胀改善情况;复制冰醋酸致小鼠毛细血管通透性增加模型,以上实验均于皮损约4cm2处外用给药1 mg.g-1。取SD大鼠60只,随机分为6组,对照组、模型组、氯雷他定组、蛇黄乳膏各浓度组,给药5 d,用0.1%磷酸组胺复制大鼠皮肤通透性增高模型;取豚鼠65只,分5组,外用皮损3 cm2给药5 d,用磷酸组胺致豚鼠瘙痒统计致痒浓度值。通过以上实验来探讨蛇黄乳膏止痒作用的相关机制。结果:蛇黄乳膏可显著抑制DNFB所诱导迟发性超敏反应,但对胸腺指数以及脾脏指数影响不明显;蛇黄乳膏可直接对抗组织胺所诱导的瘙痒反应(P<0.01),并可降低磷酸组胺所致皮肤通透性增加(P<0.01);对二甲苯直接刺激血管扩张所致的炎症、冰醋酸通过刺激炎症介质生成等所致的炎症反应有明显抑制作用(P<0.01),但对有多种致炎因子参与的角叉菜胶所致的炎症反应作用微弱。结论:蛇黄乳膏的止痒作用与调节机体免疫功能、直接对抗组织胺、减少炎症反应等作用有关。
Objective: To explore the mechanism of anti-itchiness of eczema treated by the snake yellow cream. Methods: The mice were randomly divided into control group, model group, Aloxacin group and Astragalus cream group, and the mice were given delayed-type hypersensitivity with 2,4-dinitrofluorobenzene (DNFB) The swelling inhibition rate was calculated by swelling the auricle of mice with xylene; the improvement of paw swelling was observed by carrageenan-induced paw edema in mice; the model of capillary permeability induced by acetic acid was duplicated; Damage about 4cm2 topical administration 1mg.g-1. Sixty SD rats were randomly divided into six groups: control group, model group, loratadine group and snake yellow cream at different concentrations for 5 days. The rats were perfused with 0.1% The model of sexual enhancement was established. 65 guinea pigs were divided into 5 groups and the outer skin lesions were given 3 cm2 for 5 days. The pruritus induced by histamine phosphate was used to measure the itch concentration. Through the above experiments to explore the mechanism of anti-itch effect of the snake yellow cream. Results: The snake yellow cream could significantly inhibit the delayed hypersensitivity induced by DNFB, but had no obvious effect on the thymus index and spleen index. The snake yellow cream could directly resist the itching reaction induced by histamine (P <0.01) (P <0.01). P-xylene directly stimulated the vasodilatation caused by vasodilatation, and glacial acetic acid obviously inhibited the inflammatory reaction induced by stimulating inflammatory mediators (P <0.01). However, the carrageenan-induced inflammatory response with a variety of inflammatory cytokines was weak. Conclusion: The antipruritic effect of snake yellow cream is related to the regulation of immune function, direct antagonism of histamine, and reduction of inflammatory reaction.