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目的改进火锅底料中苏丹红Ⅰ高效液相色谱测定的样品处理方法。方法样品经乙腈提取浓缩后,以乙腈和0.1%甲酸水溶液为流动相,梯度洗脱,经C18分离后,在478 nm测定。结果苏丹红Ⅰ在0.16 mg/L~2.6 mg/L范围内线性关系良好,相关系数r为0.99995,其检出限为0.02 mg/kg;加样回收率为86.2%~110.3%;方法的相对标准偏差为2.3%。结论该方法简单、快速、准确,适用于火锅底料中苏丹红Ⅰ的测定。
Objective To improve the sample processing method of Sudan Ⅰ high performance liquid chromatography in pot bottom material. Methods After the sample was extracted by acetonitrile, the mobile phase consisted of acetonitrile and 0.1% formic acid as eluent and the gradient elution was carried out. After C18 separation, the sample was determined at 478 nm. Results Sudan Ⅰ had a good linearity in the range of 0.16 mg / L to 2.6 mg / L with a correlation coefficient r of 0.99995 and a detection limit of 0.02 mg / kg. The recovery was 86.2% -110.3%. The relative The standard deviation is 2.3%. Conclusion The method is simple, rapid and accurate and is suitable for the determination of Sudan Ⅰ in hot pot.