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目的:通过检测胃癌和浅表性胃炎组织幽门螺杆菌(Helicobacterpylori,Hp)和细胞毒素相关蛋白基因(CagA),探讨Hp及CagA基因与胃癌相关性及Hp形成胃癌的可能机制。方法:应用快速尿素酶试验和组织切片革兰染色和血清HpCagA抗体检测Hp,用PCR检测HpCagA基因。结果:慢性浅表性胃炎、胃癌组织中Hp检出率分别为45.9%和54.8%,两者差异无显著性(P>0.05),两种组织中HpCagA检出率分别为35.3%和77.5%,两者差异有显著性(P<0.005)。结论:胃癌组织与浅表性胃炎组Hp感染相比差异无显著性,胃癌组织中HpCagA基因检出率明显高于浅表性胃炎组,HpCagA基因与胃癌有一定相关性,HpCagA基因可能涉及胃癌的形成机制。
Objective: To investigate the relationship between Hp and CagA genes and gastric cancer and the possible mechanism of Hp formation in gastric cancer by detecting Helicobacter pylori (Hp) and cytotoxin related protein (CagA) in gastric cancer and superficial gastritis tissues. Methods: Rapid urease test and histological sections of Gram stain and serum HpCagA antibody detection of Hp, PCR detection of HpCagA gene. Results: The detection rates of HpCagA in chronic superficial gastritis and gastric cancer were 45.9% and 54.8% respectively, with no significant difference (P> 0.05). The positive rates of HpCagA in the two tissues were 35.3% and 77.5% , The difference between the two was significant (P <0.005). Conclusions: There is no significant difference in Hp infection between gastric cancer tissues and superficial gastritis group. The positive rate of HpCagA gene in gastric cancer tissues is significantly higher than that in superficial gastritis group. HpCagA gene is associated with gastric cancer. HpCagA gene may be involved in gastric cancer The formation mechanism.