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目的研究叶酸修饰的蟾酥提取物脂质体的制备方法及包封率测定方法。方法薄膜分散法制备叶酸修饰的蟾酥提取物脂质体,HPLC法测定蟾毒灵(BL)、华蟾毒配基(CBG)、酯蟾毒配基(RBG)含量,超速离心法测定脂质体的包封率,用透射电镜观察脂质体的外观形态,并用粒径及Zeta电位分析仪测定脂质体的粒径和zeta电位。结果叶酸修饰的蟾酥提取物脂质体中BL、CBG、RBG的包封率分别为90.62%、91.90%、95.23%,形态为粒径均匀的球形和类球形,粒径为(161±15)nm,zeta电位为(4.2±1.2)m V。结论薄膜分散法制备的叶酸修饰的蟾酥提取物脂质体处方合理,工艺可行,包封率较高。
Objective To study the preparation method of folate-modified Toadstool extract liposomes and the determination of entrapment efficiency. Methods The folate-modified Toadstool extract liposomes were prepared by thin-film dispersion method. The content of bufalin (BL), bufogenin (CBG) and resorcinidin (RBG) were determined by HPLC. The lipids were determined by ultracentrifugation The encapsulation efficiency of the liposomes was observed by transmission electron microscopy. The size and zeta potential of the liposomes were determined by particle size and Zeta potential analyzer. Results The entrapment efficiencies of BL, CBG and RBG in the folate modified toad extract were 90.62%, 91.90% and 95.23%, respectively. The morphology of the entrapped beads was spherical and spheroidal with a diameter of (161 ± 15) nm and zeta potential was (4.2 ± 1.2) mV. Conclusion The folate-modified toad extract liposome prepared by the method of membrane dispersion has reasonable prescription, feasible technology and high entrapment efficiency.