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本文进一步证明草酸是一种有效的非生物诱抗剂，它能显著提高黄瓜对炭疽病的系统抗性，进而研究了草酸对黄瓜叶片中与抗病有关的过氧化物酶（ＰＯＤ）的系统诱导作用及其内在机理。结果表明：４０ｍｍｏｌ／Ｌ草酸诱导可溶态ＰＯＤ活性效果最佳；在测定时间范围内，草酸诱导的可溶态ＰＯＤ活性在第７ｄ后逐渐下降，而细胞壁离子键结合态ＰＯＤ活性被诱导后保持稳定；草酸在快带区诱导出一种新的ＰＯＤ同功酶，其Ｒｆ值为０．６３；自由基清除剂甘露醇和抗坏血酸可明显抑制草酸对可溶态ＰＯＤ活性的诱导作用；草酸诱导可溶态ＰＯＤ的作用信号可在５ｈ左右从被处理的第１片真叶传导至未处理的第２片真叶，被处理叶的保留时间越长，其诱导效果越好。
This paper further proves that oxalic acid is an effective non-biological attractant, which can significantly improve cucumber anthracnose system resistance, and then studied the oxalate cucumber leaves and disease-resistant peroxidase (POD) system Induction and its intrinsic mechanism. The results showed that 40 mmol / L oxalic acid induced the best POD activity. During the measurement time, oxalic acid-induced soluble POD activity decreased gradually after 7 days, while the cell-wall bound POD activity was induced and maintained And oxalic acid induced a new POD isoenzyme in the fast zone with an Rf value of 0.63. The free radical scavenger mannitol and ascorbic acid could significantly inhibit the induction of soluble POD activity by oxalic acid; The action signal of the dissolved POD can be transmitted from the first true leaf to the second untreated true leaf in about 5h, and the longer the retention time of the treated leaf, the better the induction effect.