由Streptomyces verticillus产生的各种博莱霉素的化学结构,都具有相同的博莱霉酸,而末端的胺部分各异,因此,博莱霉酸可用以制备各种半合成的博莱霉素。本文介绍由酶水解博莱霉素B_2制备博莱霉酸。为了寻找能水解博莱霉素B_2的微生物,接种菌种到含有10毫升灭菌培养液的50毫升摇瓶中。培养液成分如下:玉米浆0.5%,葡萄糖5%,蛋白胨0.4%,硫酸镁0.03%,磷酸二氢钾0.1%,氯化钠0.01%,氯化钙 The chemical structures of various bleomycins produced by Streptomyces verticillus all have the same Leucomachidic acid and the terminal amine moiety is different and therefore the bleomycin can be used to prepare various semisynthetic bleomycin . This article describes the preparation of zeaxanthin by enzymatic hydrolysis of bleomycin B_2. In order to find a microorganism capable of hydrolyzing bleomycin B 2, the strain was inoculated into a 50 ml shake flask containing 10 ml of sterilized culture medium. The composition of the culture solution was as follows: corn steep liquor 0.5%, glucose 5%, peptone 0.4%, magnesium sulfate 0.03%, potassium dihydrogen phosphate 0.1%, sodium chloride 0.01%, calcium chloride