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目的 分选人卵巢癌细胞系SKOV3中的侧群(side population,SP)细胞,并探讨其是否具有肿瘤干细胞的生物学特性.方法 流式细胞仪检测、分选经DNA染料Hoechst 33342染色后SKOV3中的SP细胞,并对侧群细胞(SP)与非侧群细胞(non-SP)作细胞生物学鉴定,包括增殖能力、克隆形成能力、侵袭及迁移能力、自我更新能力及细胞周期.结果 SKOV3细胞系中SP细胞比例为(1.12±0.104)％,SP细胞增殖速度快于non-SP细胞,差异有统计学意义(P＜0.05).接种相同数量的细胞,SP细胞克隆形成率高于non-SP细胞(P＜0.05).Transwell实验显示,SP细胞侵袭与迁移的细胞数与non-SP相比均明显增多(P＜0.05).SP细胞体外能分化为Non-SP细胞,具有自我更新能力.SP细胞大多处于细胞周期的G0/G1期.结论 卵巢癌细胞系SKOV3中的SP细胞具有肿瘤干细胞的生物学特性,SP细胞表型可考虑作为富集卵巢癌干细胞样细胞的有效方法.“,”Objective To identify the existence of side population(SP) cells in human ovarian cancer cell line SKOV3 and to investigate whether SP cells have the biological characteristics of cancer stem cells.Methods SP and non-SP cells from SKOV3 were isolated by fluorescence-activated cell sorting after being stained by DNA-binding dye Hoechst 33342.Biological characteristics of SP and non-SP cells were evaluated,such as proliferation ability,colony-forming efficiency,invasion and migration ability,self-renewal ability and cell cycle.Results SP cells proportion in SKOV3 cell line was (1.12±-0.104)％.The proliferation ability of SP cells was obviously greater than that of non-SP cells(P＜0.05).The colony-forming efficiency of SP cells was also higher than that of non-SP cells(P＜0.05).SP cells had a stronger ability of invasion and migration compared with nonSP cells(P＜0.05).SP cells could differentiate into non-SP cells in vitro.Most of SP cells were in the G0/G1 phase.Conclusion SP cells in SKOV3 exhibit biological characteristics of cancer stem cells.The phenotype of SP cells could be used as a potentially useful tool for enriching ovarian cancer stem cells.