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目的研究核桃青皮体外清除自由基的活性。方法采用清除2,2-二苯基苦味酰基苯肼基(DPPH)和2,2’-连氮基-双-(3-乙基苯并噻唑啉-6-磺酸)二铵盐(ABTS)自由基的方法,对核桃青皮乙醇提取物不同极性部位进行清除自由基能力的综合评价。结果石油醚、氯仿、乙酸乙酯、正丁醇、水萃取核桃青皮清除DPPH自由基的IC50分别为27.0、19.0、5.6、8.8、44.0 mg.L-1,清除ABTS自由基的IC50分别为114.0、82.6、13.6、32.0、302.0 mg.L-1。清除自由基的能力依次为:乙酸乙酯部位>正丁醇部位>氯仿部位>石油醚部位>水部位,其中,乙酸乙酯部位清除DPPH和ABTS自由基的能力高于同浓度的Vit C和Vit E。结论核桃青皮提取液的乙酸乙酯、正丁醇萃取部位具有很好的清除自由基活性,前者活性强于后者,是天然抗氧化物质的良好来源。
Objective To study the activity of scavenging free radicals on Walnut Peel in vitro. Methods The biodegradation of 2,2-diphenyl picrylhydrazyl (DPPH) and 2,2’-azino-bis- (3-ethylbenzothiazoline-6-sulfonic acid) ) Free radical method, a comprehensive evaluation of the ability of scavenging free radicals in different polar fractions of walnut green peel ethanol extract. Results The IC50 of scavenging DPPH free radicals for petroleum ether, chloroform, ethyl acetate, n-butanol and water was 27.0, 19.0, 5.6, 8.8 and 44.0 mg.L-1, respectively. The IC50 of scavenging ABTS free radicals was 114.0 , 82.6, 13.6, 32.0, 302.0 mg.L-1. The ability of scavenging free radicals was as follows: ethyl acetate> n-butanol> chloroform> petroleum ether> water. The ability of ethyl acetate to scavenge DPPH and ABTS was higher than that of vitamin C and Vit E. Conclusion The extract of walnut green peel has good activity of scavenging free radicals on the ethyl acetate and n-butanol extracts. The former is more active than the latter and is a good source of natural antioxidants.