论文部分内容阅读
目的了解乌鲁木齐市住院儿童病毒性腹泻的流行现状。方法 2010年1-12月采集乌鲁木齐市379例腹泻住院患儿粪便标本,用酶联免疫吸附试验(ELISA)、反转录-聚合酶链反应(RT-PCR)和聚合酶链反应(PCR)检测5种常见腹泻病毒。结果 229份(60.4%)粪便标本检出至少1种病毒。轮状病毒、诺如病毒、星状病毒、肠道腺病毒和札如病毒检出率分别为45.6%、14.2%、4.2%、3.2%和1.1%。混合感染共26例,以轮状病毒和诺如病毒组合占首位(50.0%,13/26)。1-3月和10-12月是轮状病毒流行高峰期;诺如病毒流行主要发生在10月。轮状病毒感染平均年龄小于诺如病毒感染平均年龄(P=0.002),肠道腺病毒和札如病毒感染全部发生于<2岁儿童。结论轮状病毒仍是2010年乌鲁木齐市儿童病毒性腹泻的最主要的病原体,其次是诺如病毒;混合感染占一定比例,在以后的流行病学及病原学研究中应该更加关注混合感染的出现。
Objective To understand the prevalence of viral diarrhea in hospitalized children in Urumqi. Methods Stool samples of 379 hospitalized children with diarrhea in Urumqi were collected from January to December in 2010. ELISA, reverse transcription-polymerase chain reaction (PCR) and polymerase chain reaction (PCR) Detection of 5 common diarrhea virus. Results 229 (60.4%) stool specimens detected at least 1 virus. The detection rates of rotavirus, norovirus, astrovirus, enteric adenovirus and zarirus were 45.6%, 14.2%, 4.2%, 3.2% and 1.1% respectively. Twenty-six cases were mixed with rotavirus and norovirus combination (50.0%, 13/26). January-March and October-December are the peak rotavirus epidemic; norovirus epidemics occur mainly in October. The average age of rotavirus infection was less than the mean age of Norovirus infection (P = 0.002), and intestinal adenovirus and Sapporo virus infection all occurred in children <2 years of age. Conclusions Rotavirus is still the most important causative agent of viral diarrhea in children in Urumqi in 2010, followed by Norovirus. The mixed infection accounts for a certain proportion, and more attention should be paid to the occurrence of mixed infection in future epidemiological and etiologic studies .