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利用筛选出的31对SRAP引物,对33份玉米自交系进行PCR扩增,采用PopGene 1.23、Structure2.3.3等软件完成玉米自交系遗传多样性和群体结构剖析,为自交系合理利用和杂交组配提供理论依据。结果显示,31对SRAP引物共检测出196个等位变异,平均6.32个;多态性比率40.00%~70.59%,平均为53.08%;基因多样性为0.2156~0.8854,平均为0.5495;PIC为0.1809~0.8976,平均为0.5507。结构分析表明,K=4时,△K值最大,即这些自交系可以划分成4个类群,依次为Reid、旅大红骨、塘四平头与PB群,新选自交系也相应地被划分到这四大类群里,没有独立成群。4个类群中,塘四平头群与旅大红骨群的遗传关系最近,与Reid群遗传关系最远。从系谱的亲缘关系分析,大部分已知自交系其SRAP聚类结果与系谱追踪结果有较好的一致性。
Thirty-three maize inbred lines were amplified by PCR using 31 pairs of SRAP primers selected. The genetic diversity and population structure of maize inbred lines were analyzed by software PopGene 1.23 and Structure 2.3.3, Hybridization to provide a theoretical basis. The results showed that 196 alleles were detected in 31 pairs of SRAP primers with an average of 6.32. The average polymorphism rate was 40.00% ~ 70.59% with an average of 53.08%. The genetic diversity was 0.2156 ~ 0.8854 with an average of 0.5495. The PIC was 0.1809 ~ 0.8976, averaging 0.5507. Structural analysis showed that △ K value was the highest when K = 4, that is, these inbred lines could be divided into four groups, followed by Reid, Ludahongtang, Tongsipingtou and PB groups, and the new selected inbred lines were also correspondingly Divided into these four groups, there is no independent group. Among the four taxa, the genetic relationship between Tongsipingtou Group and Luda Red Bone is the most recent, and the genetic relationship with Reid is the farthest. From the genetic analysis of pedigree, most of the known inbred lines have good agreement with pedigree tracking results in SRAP clustering results.