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目的 为了减少自体骨髓移植的复发率而探索一种理想的骨髓净化方案。方法 用白细胞介素-2（IL-2）、干扰素-α2a （IFN-α2a）分别净化15例急性髓细胞性白血病（AML）骨髓3d,检测净化前和净化后骨髓粒巨噬细胞集落（CFU-GM）、红系祖细胞集落（CFU-E）、红系爆式集落（BFU-E）、混合细胞集落（CFU-MIX）、白血病祖细胞集落（CFU-L）以及骨髓单个核细胞CD33、CD38、CD34的阳性表达率,并用MTT法测定IL-2、IFN-α2a对9例原代AML骨髓细胞的毒性作用,从而观察其对白血病细胞和正常干/祖细胞的影响。结果（1）IL-2、IFN-α2a在净化过程中对CFU-GM、CFU-E、BFU-E、CFU-MIX无影响;（2）IL-2、IFN-α2a均能明显降低CD33、CD38的阳性率,增加CD34的阳性率（P<0.05）,二者联合作用更强（P<0.01）;（3）在净化处理前,15例AML患者CFU-L（集落数/2×105MNC）为130.47±17.49,在净化处理后,IL-2组为14.32±6.24,IFN-α2a组为24.68±8.46,IL-2联合IFN-α2a净化组则没有培养出 CFU-L,未加细胞因子净化对照组为85.12±18.86,各净化组与对照组比较差异均有显著性（P<0.01）,二者联合作用更显著（P<0.01）;（4）IL-2、IFN-α2a对AML骨髓中原代白血病细胞的细胞毒作用（％）分别为50.2±8.4、32.41±5.6,二者联合应用则为72.4?
Objective To explore an ideal bone marrow purification protocol in order to reduce the recurrence rate of autologous bone marrow transplantation. Methods 15 cases of acute myeloid leukemia (AML) bone marrow were purified by interleukin-2 (IL-2) and interferon-α2a (IFN-α2a) respectively. The bone marrow granulocyte colony before and after purification CFU-GM, CFU-E, BFU-E, CFU-MIX, CFU-L and bone marrow mononuclear cells The positive rates of CD33, CD38 and CD34 were determined. The cytotoxicity of IL-2 and IFN-α2a on 9 cases of primary AML myeloid cells were determined by MTT assay to observe the effect on the leukemic cells and normal stem / progenitor cells. Results (1) IL-2 and IFN-α2a had no effect on CFU-GM, CFU-E, BFU-E and CFU-MIX during the purification process; (P <0.01); (3) CFU-L (number of colonies / 2 × 105MNC) in 15 AML patients before purification ) Was 130.47 ± 17.49. After decontamination, IL-2 group was 14.32 ± 6.24, IFN-α2a group was 24.68 ± 8.46, CFU-L was not cultured in IL-2 plus IFN-α2a group, no cytokines (P <0.01). (4) The effect of IL-2 and IFN-α2a on the expression of AML in the purified group was 85.12 ± 18.86, the difference between the purified group and the control group was significant The cytotoxicity of primary leukemia cells in bone marrow (%) was 50.2 ± 8.4,32.41 ± 5.6, respectively, and the combination of the two was 72.4%.