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为探索一种检测人、食肉动物和啮齿动物体内鼠疫抗体的方法,我们对酶联免疫吸附试验(ELISA)进行了研究。结果表明,ELISA 可以代替标准的被动血凝试验(PHA),用于诊断人类鼠疫、鼠疫控制和监测活动。与 PHA 不同,ELISA 可用于测定病人血清和其他样品中的特异免疫球蛋白(IgG、IgM 等)。本文报告用 ELISA做鼠疫血清学诊断在某些方面比 PHA 先进,同时也对上述两种检验技术的优点和局限性进行了比较。
To explore a method for the detection of plague antibodies in humans, carnivores and rodents, we examined the enzyme linked immunosorbent assay (ELISA). The results show that ELISA can replace the standard passive coagulation test (PHA), for the diagnosis of human plague, plague control and monitoring activities. Unlike PHA, ELISA can be used to determine specific immunoglobulins (IgG, IgM, etc.) in patient serum and other samples. In this paper, we report that the serological diagnosis of plague using ELISA is advanced in some respects to PHA, and the advantages and limitations of these two assays are also compared.