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肌酐测定的化学反应有:肌酐和碱性苦味酸盐反应,萘醌反应,硝基苯甲醛反应,二硝基苯甲酸反应,纳氏试剂反应等。其中以碱性苦味酸盐反应最常用。雅菲(Ja-ffe′)氏于1886年介绍肌酐和苦味酸在碱性溶液中呈色反应时就指出它的非特异性,当时认为丙酮有干扰。以后其它研究者指出葡萄糖、双醋酸酯、丙酮酸,抗坏血酸、胍基醋酸内酰胺、氨基马尿酸盐、蛋白质、果糖以及邻苯二酚,对苯二酚、间苯二酚等亦有干扰。
Creatinine determination of the chemical reactions are: creatinine and alkaline picrate reaction, naphthoquinone reaction, nitrobenzaldehyde reaction, dinitrobenzoate reaction, Nessler reagent reaction. One of the most commonly used alkaline picrate reaction. Jaffe’s introduction of creatinine and picric acid in 1886 led to its nonspecificity when it was colored in an alkaline solution when acetone was considered to interfere. Other researchers later pointed out that glucose, diacetate, pyruvate, ascorbate, guanidinolactam, aminoglycoside, protein, fructose, and catechol, hydroquinone, resorcinol and the like also interfere .