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目的：将荧光染色体原位抑制（ＣＩＳＳ）杂交法运用于急性早幼粒细胞白血病（ＡＰＬ）染色体畸变的检测，提高异常核型的检出率。方法：以生物素标记的人类１５号染色体特异的ＤＮＡ为探针，对１０例ＡＰＬ患者骨髓中期分裂相进行杂交，所有病例均进行Ｇ显带分析。结果：ＣＩＳＳ杂交法异常核型的检出率较传统Ｇ显带高或在Ｇ显带无法分析时，提供结果。结论：ＣＩＳＳ杂交结果更全面反映白血病细胞的遗传学特性，在检测细微结构异常、复杂易位以及残留白血病细胞等方面，具有快速、敏感、特异及对嵌合细胞群体可定量分析的优点。
Objective: To apply fluorescent chromosome in situ suppression (CISS) hybridization to the detection of chromosomal aberrations in acute promyelocytic leukemia (APL) and to increase the detection rate of abnormal karyotypes. METHODS: Biotin-labeled human chromosome 15 specific DNA was used as a probe to hybridize 10 cases of APL patients with metaphases of bone marrow. G banding analysis was performed in all cases. RESULTS: The detection rate of abnormal karyotypes by the CISS hybridization method was higher than that of the traditional G banding or provided results when the G banding could not be analyzed. Conclusion: The results of CISS hybridization reflect the genetic characteristics of leukemia cells more comprehensively, and have the advantages of rapid, sensitive, specific and quantitative analysis of chimeric cell populations in detecting subtle structural abnormalities, complex translocations and residual leukemia cells.