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采用已筛选出的多态性较好的分布于六倍体小黑麦21对染色体上的100对SSR引物对从国际玉米小麦改良中心(CIMMYT)引进的339份六倍体小黑麦进行了遗传多样性分析。结果显示,100对引物共检测出323个等位变异,变幅是1~6个,平均等位变异丰富度为3.23;多态信息含量(PIC)的变幅为0~0.748,平均多态信息含量为0.465;平均遗传多样性指数(H’)为0.1439,说明供试的339份六倍体小黑麦品种的SSR遗传多样性较为丰富。同时基于Nei’s遗传距离对339份材料进行聚类分析,可以将供试材料分为6个类群。其中,第Ⅵ类群与其他类群的遗传距离较大、遗传差异较明显。研究结果为将六倍体小黑麦的优良基因应用于普通小麦的遗传改良提供了理论依据。
A total of 100 pairs of SSR primers distributed on 21 pairs of chromosomes of hexaploid Triticeae were screened for 339 hexaploid tritree plants introduced from International Maize Improvement Center for Wheat (CIMMYT) Genetic diversity analysis. The results showed that a total of 323 alleles were detected with 100 pairs of primers, with amplitudes ranging from 1 to 6 with a mean allele-variant abundance of 3.23. The polymorphism information content (PIC) ranged from 0 to 0.748 and the average polymorphism The information content was 0.465, and the average genetic diversity index (H ’) was 0.1439, which indicated that SSR genetic diversity of 339 hexaploid tritium cultivars tested was rich. Based on Nei’s genetic distance, 339 materials were clustered and divided into 6 groups. Among them, the Ⅵ group with other groups of genetic distance larger, more obvious genetic differences. The results provide a theoretical basis for applying the good genes of hexaploid triticale to the genetic improvement of common wheat.