为了排除在体神经、体液因素的影响,固定心脏负荷状态、测取EF,LVEDV等指标.同时观察心脏收缩、舒张性能、顺应性和心泵功能,建立了大鼠离体作功心脏灌流模型。实验表明,该模型稳定;对负荷、药物反应灵敏,缺血、重灌实验所得结果与文献一致。此模型可用于生理、生化、药理、病生等学科的实验研究。 In order to rule out the influence of somatic and humoral factors, the cardiac load was fixed and EF, LVEDV and other indexes were measured.At the same time, cardiac systolic and diastolic function, cardiac compliance and heart pump function were observed to establish a model of rat cardiac perfusion . Experiments show that the model is stable; the load, drug responsive, ischemia, reperfusion test results obtained with the literature. This model can be used in the physiological, biochemical, pharmacological, disease and other disciplines of experimental research.