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目的 探讨不同 +GZ重复暴露后 ,大鼠不同脑区热休克蛋白 - 70 (HSP70 )表达强度和时程的变化规律 ,及其与正加速度致脑损伤的相关性。 方法 将雄性 SD大鼠随机分为对照组、+2 GZ、+6 GZ和 +10 GZ暴露组。分别在暴露后 6 h、1d、2 d、4d和 6 d处死取脑 ,利用 HE和免疫组织化学染色 ,观察 HSP70在鼠脑不同部位的表达 ,及脑神经元形态学和血脑屏障通透性的变化。 结果 +GZ 重复暴露可诱导 HSP70的表达 ,其表达强度、持续时间和部位随 G值而异。低 G值(+2 GZ)下 ,表达强度弱 ,持续时间短 ,但范围较广 ;高 G值 (+10 GZ)下 ,表达仅局限在下丘脑和梨状皮层等部位 ,呈中等强度反应 ;中等强度 G值 (+6 GZ)下 ,表达范围广 (在皮层、海马、丘脑等部位均有HSP70较强反应 ) ,持续时间长。 结论 +6 GZ诱导的 HSP70表达最强 ,持续时间最长 ,为研究热休克蛋白对正加速度致脑损伤的保护作用 ,提供了实验依据
Objective To investigate the changes of heat shock protein 70 (HSP70) expression intensity and time course in different brain regions after repeated + GZ exposure in rats and its correlation with positive acceleration brain injury. Methods Male SD rats were randomly divided into control group, +2 GZ, +6 GZ and +10 GZ exposure groups. The brains were sacrificed at 6 h, 1 d, 2 d, 4 d and 6 d after exposure respectively. HE staining and immunohistochemical staining were used to observe the expression of HSP70 in different parts of the brain and the neuronal morphology and blood-brain barrier permeability Sexual changes. Results + GZ repeated exposure induced the expression of HSP70, whose expression intensity, duration and location varied with G value. Under the low G value (+2 GZ), the expression intensity was weak and the duration was short, but the range was wide. Under the high G value (+10 GZ), the expression was only confined to the hypothalamus and piriform cortex, Under the moderate G value (+6 GZ), the expression range was wide (strong HSP70 was observed in the cortex, hippocampus and thalamus) for a long time. Conclusion +6 GZ induced the strongest expression of HSP70, the longest duration, in order to study the protective effect of heat shock protein on the brain injury induced by positive acceleration, providing experimental evidence