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被大肠杆菌噬菌体G4侵染后细胞的溶菌产物中发现了旦白质K。决定它的基因被命名为基因K.剑桥大学的肖(Shaw)及其合作者采用了桑格(Sanger)等人的一种新的顺序分析技术,对基因K进行DNA顺序分析,发现了重迭基因的第三个例证。基因K与其它基因重迭在一起:它的5′近端与基因A的最后86个核苷酸重迭;它的3′近端与基因C的开始89个核昔酸重迭。在这两个重迭的部位,DNA的密码容量被使用在三种阅读框架。例如,在TGATG顺序中,A既被认为是基因K的起始密码子ATG的一部分,又是基因B的终止密码子TGA的一部分,同时还是基因A中天冬氨酸
Proteins were found in the lysates of cells infected with E. coli phage G4. The gene that determines it is named gene K. Shaw of the University of Cambridge and co-workers used a new sequence analysis technique from Sanger et al. To perform a DNA sequence analysis of gene K and found that heavy The third example of Diego gene. The gene K overlaps with other genes: its 5 ’proximal end overlaps with the last 86 nucleotides of gene A; its 3’ proximal end overlaps with the beginning of 89 nucleotides of gene C. In these two overlapping parts, the DNA password capacity is used in three reading frames. For example, in the TGATG sequence, A is considered both as part of the start codon ATG of gene K and as part of the stop codon TGA of gene B, and also aspartate in gene A