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该研究运用代谢组学方法,研究麻黄细辛附子汤(MXF)干预的流感小鼠血清中内源性代谢物的变化,寻找潜在生物标志物及相关的代谢通路,探讨MXF的药效及作用机制。实验选用昆明(KM)小鼠36只,随机分为正常组、流感组、MXF组,使用流感病毒H1N1滴鼻感染,建立流感小鼠模型;MXF组使用药物干预,其他组给予等体积的生理盐水,每日1次,连续6 d,记录小鼠的体重、肛温等药理指标,末次给药24 h后收集血清样本,采用HPLC-TOF-MS检测。结果显示,与正常组相比,流感组小鼠体重、肛温均出现不同程度下降,肺指数和死亡率明显升高(P<0.05),MXF组各药理指标出现良好的回调趋势;血清样本采集的原始数据,使用R语言,MPP,SIMCA-P等软件进行处理,发现3组小鼠血清中14种内源性物质发生显著性变化(P<0.05),经MXF干预后代谢物水平均出现不同程度的回调;进一步利用Met PA数据平台分析发现,14种代谢物涉及的通路包括糖代谢、花生四烯酸代谢、甘油磷脂和鞘脂代谢等。通过对这些差异性代谢物生理功能的分析,并结合测定的宏观药理指标的分析,推测MXF可能从改善糖代谢,调节花生四烯酸代谢、甘油磷脂和鞘脂代谢等多途径,发挥其药效作用。
In this study, metabolomics was used to study the changes of endogenous metabolites in the serum of influenza mice intervened by Ephedra asarum Aconite Decoction (MXF), searching for potential biomarkers and related metabolic pathways, and exploring the efficacy and effects of MXF mechanism. Thirty-six Kunming mice were randomly divided into normal group, flu group and MXF group. Flu mice were infected with influenza virus H1N1. The mice in MXF group were treated with drugs. The other groups were given equal volume of physiology Saline, once a day for 6 days. The body weight, rectal temperature and other pharmacological indicators were recorded. Serum samples were collected 24 h after the last administration and detected by HPLC-TOF-MS. The results showed that compared with the normal group, the body weight and rectal temperature in the flu group decreased to different extents, the lung index and mortality were significantly increased (P <0.05), and the pharmacological indicators in MXF group showed a good trend of callback; serum samples The collected raw data were processed by R language, MPP, SIMCA-P and other software and found that 14 kinds of endogenous substances in the three groups of mice had significant changes (P <0.05). The levels of metabolites With different degrees of callback. Further analysis using Met PA data platform found that the 14 metabolites involved in the pathway include glucose metabolism, arachidonic acid metabolism, glycerophospholipids and sphingolipid metabolism. Through the analysis of the physiological functions of these different metabolites and the analysis of the macroscopic pharmacological indicators, it is speculated that MXF may play its role in improving metabolism of sugar, regulating the metabolism of arachidonic acid, glycerophospholipid and sphingolipid metabolism, etc. Effective effect.