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目的探讨外阴鳞状细胞癌(鳞癌)组织中染色体变异与人乳头状瘤病毒(HPV)感染状态的关系。方法应用比较基因组杂交(CGH)技术和PCR技术分别检测21例外阴鳞癌患者癌组织中染色体的变异情况和HPV感染状态,并分析两者间的关系。结果每例患者的外阴鳞癌组织中染色体均有不同程度的变异,包括染色体全部或部分的扩增或丢失,每例患者平均有4·9处(102/21),其中丢失多于扩增,分别为2·6处(54/21)和2·3处(48/21)。21例患者中,根据染色体变异部位的不同,染色体扩增率依次为3q占43%(9/21)、8q占38%(8/21)、12q占33%(7/21)和9p占19%(4/21),染色体丢失率依次为4p占52%(11/21)、3p占43%(9/21)和9p占10%(2/21)。在染色体3q及12q部位,HPV阳性的外阴鳞癌组织染色体扩增率(分别为73%、64%)均明显高于HPV阴性者(分别为10%、0,P<0·01);而在染色体8q部位,HPV阴性的外阴鳞癌组织染色体扩增率(70%)明显高于HPV阳性者(9%,P<0·01)。结论外阴鳞癌组织中存在染色体的扩增或丢失。染色体3q、12q的扩增与外阴鳞癌患者感染HPV有关。
Objective To investigate the relationship between chromosomal aberrations and human papillomavirus (HPV) infection in vulvar squamous cell carcinoma (squamous cell carcinoma). Methods Chromosome variation and HPV infection in 21 cases of vulvar squamous cell carcinoma were detected by comparative genomic hybridization (CGH) and PCR. The relationship between them was analyzed. Results In each case, the variation of chromosomes in vulvar squamous cell carcinoma was found in all patients, including the amplification or loss of chromosomes in whole or in part. The average number of cases in each group was 4.9 (102/21), with more loss than amplification , Which were 2.6 (54/21) and 2.3 (48/21) respectively. According to the chromosomal aberrations, chromosome amplification rates were 3q (43/9), 8q (38/8), 12q (33/21) and 9p 19% (4/21). The chromosome loss rates were 4p in 52% (11/21), 3p in 43% (9/21) and 9p in 10% (2/21). On chromosome 3q and 12q, HPV-positive vulvar squamous cell carcinoma had a significantly higher rate of chromosome amplification (73% and 64%, respectively) than HPV-negative patients (10% and 0%, respectively; P <0.01) On the chromosome 8q, HPV-negative vulvar squamous cell carcinoma had a significantly higher rate of chromosome amplification (70%) than HPV-positive (9%, P <0.01). Conclusion There are chromosome amplification or loss in vulvar squamous cell carcinoma. Chromosome 3q, 12q amplification and vulvar squamous cell carcinoma in patients with HPV infection.