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目的:探讨偏头痛家兔动物模型的复制方法,从行为学方面进行评价。方法:参照大鼠的偏头痛造模方法,按照5 mg/kg(低剂量)、7.5 mg/kg(中剂量)、10 mg/kg(高剂量)的标准进行颈部皮下注射硝酸甘油注射液,观察甩头、挠头、扒笼的行为学变化,同时检测血浆一氧化氮(NO)进行客观评价。结果:(1)低剂量组偏头痛家兔模型甩头次数和扒笼次数在31~120 min时段内、挠头次数在61~150 min时段内,较正常组均有明显差异(P<0.01);(2)中剂量组偏头痛家兔模型甩头次数、挠头次数和扒笼次数在31~150 min时段内,较正常组均有明显差异(P<0.05,P<0.01);(3)低剂量组和中剂量组挠头次数在31~120 min时段内,比较均有明显差异(P<0.05,P<0.01);(4)三组不同剂量组的NO含量均较正常组高,与正常组均有明显差异(P<0.05),三组间比较无明显差异(P>0.05)。结论:5 mg/kg以上剂量均可诱发偏头痛发作,以7.5 mg/kg剂量较为适宜,注射后30 min起效,维持2 h,挠头是偏头痛家兔模型疼痛的突出表现,血浆NO升高是偏头痛家兔模型复制的评价指标。
Objective: To explore the method of replicating animal model of migraine in rabbits and to evaluate its behavior. Methods: According to the model of migraine in rats, subcutaneous injection of nitroglycerin into the neck was carried out according to the standard of 5 mg / kg (low dose), 7.5 mg / kg (medium dose) and 10 mg / kg Observing the behavioral changes of head shaving, scratching head and cage, and objectively evaluating plasma nitric oxide (NO). Results: (1) Within the range of 31-120 min, the frequency of scratching in the low-dose group of migraine rabbits was significantly different from that of the normal group (P <0.01) ; (2) Compared with the normal group, the number of head shaving, the number of head scratching and the number of hanging cage in the mid-dose migraine rabbit model were significantly different from those in the normal group (P <0.05, P <0.01) The number of scratches in the low-dose group and middle-dose group were significantly different in the period of 31-120 min (P <0.05, P <0.01). (4) The content of NO in the three groups of different dose groups was higher than that in the normal group The normal group had significant difference (P <0.05), no significant difference between the three groups (P> 0.05). CONCLUSIONS: Migraine attacks can be induced at doses above 5 mg / kg, with a dose of 7.5 mg / kg being more appropriate and effective at 30 min after injection for 2 h. Scratching is a prominent manifestation of pain in migraine rabbit models. High is a marker of migraine rabbit model replication evaluation.