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目的研究甲基汞对秀丽隐杆线虫神经元的损伤作用,观察线虫虫期、染毒方式、检测指标与时间对甲基汞神经毒性的影响。方法 L1期、L4期秀丽隐杆线虫用0~300μmol/L甲基汞液体染毒30 min或固体染毒24~72 h,于染毒开始后的24~72 h检测神经元形态及行为学指标。结果甲基汞破坏秀丽隐杆线虫多巴胺神经元与γ-氨基丁酸神经元结构的完整性;不论选用L1期还是L4期线虫,采用固体染毒还是液体染毒,甲基汞染毒后24 h即可观察到线虫神经元结构、行为功能显著改变;L1期线虫对甲基汞的毒性作用较L4期线虫敏感;身体弯曲频率、头部摆动频率是对损伤最为敏感的行为学指标。结论甲基汞损伤秀丽隐杆线虫多巴胺神经元与γ-氨基丁酸神经元;秀丽隐杆线虫能够作为甲基汞神经毒性及机制研究的模式生物。
Objective To study the effect of methylmercury (Hg) on the neurons of C. elegans, and to observe the effects of nematode stage, exposure mode, test index and time on the neurotoxicity of methylmercury. Methods The neurite morphology and behavior of Caenorhabditis elegans at 0 ~ 300μmol / L methylmercury liquid for 24 or 72 h were detected by ELISA. index. Methylmercury was used to destroy the structural integrity of dopamine neurons and γ-aminobutyric acid neurons in C. elegans. No matter whether L1 or L4 nematodes were selected, solid or liquid methylmercury h, the neuronal structure of nematode was observed and the behavioral function changed significantly. The toxicity to methylmercury in stage L1 nematode was more sensitive than that in stage L4 nematode. The body bending frequency and head swing frequency were the most sensitive behavioral indexes for injury. Conclusion Methylmercury can damage dopaminergic neurons and γ-aminobutyric acid neurons of C. elegans; Caenorhabditis elegans can serve as a model organism for studying the neurotoxicity and mechanism of methylmercury.