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本文报告了一种简便、精确地测定血中1, 25-双羟维生素D的方法。1ml血清与碱性K_2HPO_4缓冲液温育1小时后与硅藻土粉末混合,继之用二氯甲烷/丙酮(9:1)提取、分离,经高效液相色谱仪纯化,流动相为正己烷/异丙醇(9:1),经放射受体法测定1, 25(OH)_2D含量。1, 25(OH)_2D_3受体蛋白提取、制备自正常仔鸡小肠粘膜细胞。34例正常北京成人血清1.25(OH)_2D浓度平均为113.8±422pmol/L(47.4±17.6pg/ml)。
This article reports a simple and accurate method for the determination of 1,25-dihydroxyvitamin D in blood. 1 ml of serum was incubated with alkaline K 2 HPO 4 buffer for 1 hour and then mixed with diatomaceous earth powder followed by extraction with dichloromethane / acetone (9: 1), separation and purification by high performance liquid chromatography with a mobile phase of n-hexane / Isopropanol (9: 1), and the content of 1, 25 (OH) _2D was determined by radioactive receptor method. 1, 25 (OH) _2D_3 receptor protein extraction, prepared from normal small intestine mucosal cells. The average serum concentration of 1.25 (OH) _2D in 34 normal Beijing adults was 113.8 ± 422 pmol / L (47.4 ± 17.6 pg / ml).